单核细胞趋化蛋白-1对膀胱肿瘤荷瘤裸鼠的作用

The Effect of Monocyte Chemotactic Protein-1(MCP-1)in Nude Bearing Human Transitional Cell Cancer of Bladder

目的 :探讨不同浓度单核细胞趋化因子 (MCP - 1)对EJ荷瘤裸鼠的抑瘤作用。方法 :用EJ细胞接种于 2 0只裸鼠 (BALB/c -nu/nu) ,2 1至 2 8天后接种成功 ,随机对照分组 ,用MCP - 1腹腔连续注射 11天观察MCP- 1对荷瘤裸鼠的作用。结果 :用MCP - 10 5ng/ml及MCP - 15ng/ml腹腔连续注射 11天 ,每天 1次 ,1次 0 2ml。与生理盐水对照组相对比肿瘤体积和肿瘤重量以及单核细胞密度有显著差异 ,肿瘤重量抑制率分别为 4 0 %与4 2 % ,肿瘤体积抑制率分别为 4 8%与 5 0 % ,病理切片单核细胞密度高于对照组 (P <0 0 1) ,但用药两组间对比无显著差异。结论 :在裸鼠动物实验中 ,MCP - 1可抑制肿瘤的生长 。

Objective:To investigate the antitumor effect of different concentration monocyte Chemotactic Protein-1(MCP-1)in nude bearing human transitional cell cancer of bladder.Methods:The mice bearing tumor were randomly divided into 4 groups.Different concentration MCP-1 were injected via peritoneal cavity for 11 consecutive days.Results:There was significant difference in tumor volume and tumor weight between treatment group and control group(P<0.01).The tumor suppressing rate for tumor volume and weight were 48%,40% after injection of 0.2ml MCP-1 with 0.5ng/ml,and were 50%,42% after injection of 0.2ml MCP-1 with 5ng/ml.The quantity monocyte of tumor were significant increased in two treatment groups compared with control group(P<0.01).Conclusion:The growth of tumor was inhibited by MCP-1 in a certain concentration.Enhancement of the quantity and killing ability of monocyte may be involved in this mechanism.