摘要
目的 研究不同剂量褐藻糖胶对受γ射线照射大鼠免疫功能影响的作用机理。方法不同剂量褐藻糖胶经口给予实验组大鼠 ,连续给药 10d后行一次性 6 0Coγ射线照射 ,18h后测定各组大鼠体液免疫、细胞免疫和非特异性免疫相关指标 ,并测定大鼠脾淋巴细胞凋亡率。结果 10 0mg kg褐藻糖胶即能显著提高照射后大鼠T、B淋巴细胞增殖能力、巨噬细胞吞噬功能、血清溶血素含量 ,增强大鼠迟发性超敏反应 ,抑制照射后脾淋巴细胞凋亡 ,并呈现剂量 效应关系 ,与阳性对照组比较差异有非常显著性 (P <0 0 1)。结论 褐藻糖胶是一种抗照射免疫调节剂 ,对照射后大鼠的免疫系统具有恢复作用 。
Objective To investigate the effect of immunologic function of fucoidan at different doses given preventively to rats exposed to γ-rays and its mechanism. Methods The Wistar rats were given fucoidan at different doses o rally for 10 d before whole-body irradiation with 60 Co γ-rays.The r elated indexes of humoral,cellular and nonspecific immunologic functions and apo ptosis ratio of lymphocytes were measured 18 hs later. Results Fucoidan at 100 mg/kg was able to significantly enhance the ability of prolifera tion responses of T,B lymphocytes,the number and phagocytosis of macrophage,seru m hemolysin contents,the delayed type hypersensitivity response (DTH) of rats ex posed to γ-rays and to reduce apoptosis ratio of lymphocytes in irradiated rat s.There were dose-effect relationships in these observation indexes of the fuco idan groups.There were significant differences between these indexes of the fuco idan groups and those of the positive control group (P<0 01). Conclusion These results indicate that fucoidan as an immunoregulatory chemical could promo te the recovery of immunologic function in irradiated rats.The mechanism is asso ciated with the arrest of lymphocyte apoptosis by fucoidan.;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2003年第6期430-432,共3页
Chinese Journal of Radiological Medicine and Protection
基金
湖北省科技厅重大攻关资助项目(2 0 0 1AA30 7B)