HIV抗体酶联免疫诊断试剂检测不同基因型抗体的研究

Capacity of HIV enzyme immunoassay diagnostic kits to detect antibodies against different genotypes of HIV

目的 分析不同HIV抗体酶联免疫诊断试剂对检测HIV不同基因型抗体的情况。方法对不同地区的20份HIV抗体阳性样品中HIV核酸进行扩增,对PCR产物进行测序并进行基因型别分析。用不同试剂对系列稀释的不同基因型样品进行检测。结果 20份样品均为HIV RNA阳性,其中9份样品为HIV B亚型,9份样品为HIV C或BC重组,2份为HIV AE重组。不同试剂对HIV不同基因型抗体的检测灵敏度无明显差异。结论 我国主要的商业化HIV抗体诊断试剂产品检测不同基因型抗体的能力无明显差异。

Objective To investigate the capacity of commercial HIV enzyme immunoassay (EIA) diagnostic kits to detect antibodies against different genotypes of HIV. Methods HIV RNA was detected with RT-PCR from samples positive for HIV antibody. The purified PCR products were sequenced directly and the genotypes of HIV from samples were analyzed. The samples for each genotype of HIV were diluted and the diluted samples were detected with different HIV EIA diagnostic kits. Results All 20 samples positive for HIV antibody were also positive for HIV RNA; 9 of 20 isolates were genotype B, 9 of them were genotype C or CRF BC, 2 of them were CRF AE. The sensitivity of different HIV EIA diagnostic kits to detect antibodies against different genotypes of HIV was not significantly different. Conclusion The capacity of commercial HIV diagnostic kits to detect antibodies against different HIV genotypes may not be significantly different.