缬沙坦和螺内酯对自发性高血压大鼠心肌整和素β_1的作用

The Suppressing Effect of Valsartan and Spironolactone on Expression of Integrinβ_1 in Cardiac myocytes Spontaneously Hypertensive Rats

目的 比较自发性高血压大鼠 (SHR)和WKY心肌整和素β1的表达以及缬沙坦和螺内酯对SHR整和素 β1的影响。方法 将 18只 6周龄SHR随机分成 3组 ,每组 6只。其中 2组分别灌喂缬沙坦 2 0mg·kg- 1·d- 1,和螺内酯 10mg·kg- 1·d- 1,另一对照组给正常饮水 ,并与雄性同周龄WKY6只比较。实验期 14周 ,免疫组化法检测四组大鼠心肌整和素 β1的表达。结果 SHR对照组心肌整和素 β1表达明显高于WKY组 ,和SHR对照组比较缬沙坦组整和素 β1的表达下降 (P <0 0 5) ,而螺内酯组整和素 β1的表达无显著改变 ;两治疗组血压、LVM/BW以及心肌纤维化程度均显著低于SHR组 (P <0 0 5) ,而且两治疗组间比较缬沙坦组血压和心肌纤维化程度降低更明显 (P <0 0 5)。结论 缬沙坦除降低血压外 ,还能抑制整和素 β1表达 ,而螺内酯对整和素β1的表达无明显抑制作用 ;而缬沙坦对心肌纤维化的抑制作用优于螺内酯。提示整和素

Objective To explore the surpressing effect of valsartan an d spironolactone on expression of integrin β\-1 in cardiac myocytes in spontaneously hypertensive rats. Methods Eighteen 6-week-old SHRs were randomly to receive valsartan (20 mg·kg -1 ·d -1 ) or spironolactone (10 mg·kg -1 ·d -1 ).\ 6 SHR and 6 WKY rats served as control. After 14 weeks, expression of integrin β 1 in cardiac myocytes were studied by immunohistochemistry. Results The ex pression of integrin β 1 were higher in SHR than in WKY group(P<0 05).\ Losartan inhibit the expression of integrin β 1, while no change in integrin by spironolactone was found.\ Both valsartan and spironolactone decreased blood pressure, left vnticular mass to body weight ratio(LVM/BW) and the extent of cardiac fibrosis. Conclusion Although both valsartan and spironolactone decreased blood pressure, the inhibition effect on the integrin β\-1 in cardial myocytes was ma nifested only by valsartan treatment. Valsartan was more effective i n attenuating myocardial fibiosis than that by spironolactone. Integrin β 1 may play a role in hypertensive cardiac fibrosis.