摘要
目的 探讨脑挫伤后细胞凋亡相关基因Bcl -2、Fas表达的变化规律推断脑损伤时间。 方法 通过组织学和免疫组织化学法 (SP法 )观察、分析大鼠脑挫伤早期脑组织病理形态学改变及Bcl -2、Fas表达的变化规律。 结果 伤后 3 0min在脑挫伤周围神经细胞可见Bcl -2、Fas阳性表达 ,以后呈上升趋势 ,并且范围逐渐扩大 ,其中Bcl -2蛋白表达在 6h达高峰 ,随着呈下降趋势 ,8h后可见典型的凋亡神经细胞。
Objective To investigate the relationship between the expressive variety of apoptosis-related gene Bcl-2.Fas and the brain injury time after cerebral contusion. Methods The morphologia feature of brain tissue and the expression of Bcl-2.Fas were microscopically observed by immunohistochemical method. Result The expression of apoptosis-related gene was noted after 30 minutes and keep increasing subsequently.The expression of Bcl-2 reached the maximum level at 6 hours after brain trauma and then declined.Typical apoptotic cells were seen 8 hours after cerebral contusion. Conclusion Detecting the expression of Bcl-2 and Fas would be of valuable in the distinguishment of promortem and postmortem injury,and the estimation of brain injury time during the earlier period after cerebral contusion.
出处
《实用预防医学》
CAS
2003年第6期904-906,共3页
Practical Preventive Medicine
关键词
凋亡相关基因
推断脑损伤时间
组织学
免疫组织化学
Apoptosis-related gene
To investigate the brain injury time
Tissuechemistry
Immunohistochemistry
