458株临床分离菌的种类分布及耐药性分析

458 Clinical Pathogenic Strains: Distribution and Antibiotic Resistance

目的 通过对 45 8株临床分离菌的细菌种类分布及耐药性研究 ,指导临床合理用药。 方法 细菌鉴定采用常规方法 ,药敏试验采用纸片扩散法 (K -B法 ) ,并按 1999年美国临床实验室标准化委员会 (NCCLS)标准进行分析和质控。 结果 最常见的临床分离菌依次为大肠埃希氏菌、绿脓假单胞菌、凝固酶阴性的葡萄球菌、克雷伯氏菌、金黄色葡萄球菌、志贺氏菌、肠杆菌属细菌等。 45 8株临床分离菌对常用较新 17种抗生素均有不同程度的耐药。 结论 临床微生物实验室应及时总结并不断监测临床分离菌对常用较新抗生素的耐药性 ,临床医生必须关注病原菌的分布及耐药情况 。

Objective To determine the distribution and drug-resistance rates of pathogenic bacteria isolated from patients so as to give a guide for reasonable application of antibiotics in clinic. Methods We used Kirby-Baner (K-B) test for antibiotics susceptivity test. The results were evaluated based on National Committee for Clinical Laboratory Standards (NCCLS) in 1999. Result Most of the strains were Escherichia coli.(16.38%, 75/458), P. aeruginosa (15.94%, 73/458), Staph. Coagulase(-) (14.41%, 66/458), Klebsiella (12.66%, 58/458), Staph. Aureus ( 11.79 %, 54/458), Shigella (8.08%, 37/458) and Enterobacter (4.59%, 21/458). A total of 458 pathogenic strains were isolated from patients resistant to 17 antibiotics with different degrees. Conclusion It is very important to summarize and monitor the antibiotic resistance of clinical pathogenic bacteria. Physicians should pay attention to the distribution and drug-resistance of pathogenic bacteria, so that antibiotics could be applied reasonably.