卡维地洛及TNF-α对内皮细胞释放t-PA和PAI-1的影响

THE EFFECTS OF CARVEDILOL AND TNF-α ON ENDOTHELIAL CELL SECRETING t-PA AND PAI-1

目的 研究卡维地洛及肿瘤坏死因子α(TNF α)对内皮细胞分泌组织纤溶酶原激活物 (t PA)和纤溶酶原激活物抑制剂 1(PAI 1)的影响。方法 培养内皮细胞株 (ECV3 0 4) ,分为TNF α刺激组 ,培养基中TNF α加至终浓度为 5、10、2 5、5 0、10 0ng/ml;卡维地洛干预组 ,培养基中加TNF α (5 0ng/ml)后加入卡维地洛 ,终浓度为 2 0、5 0、10 0、2 0 0nmol/L ,2 4h后测定上清中的组织纤溶酶原激活物 (t PA)和纤溶酶原激活物抑制剂 1(PAI 1)的含量。结果 内皮细胞在TNF α刺激 2 4h后 ,其分泌的PAI 1抗原含量与对照组比有明显升高 ,有显著性差异 (P <0 0 5 ) ,而两组间t PA含量无明显差异 (P >0 0 5 )。而卡维地洛干预组却显著降低PAI 1(P <0 0 5 ) ,对t PA含量无明显作用 (P >0 0 5 )。结论 TNF α对内皮细胞株分泌的PAI 1有显著的升高作用 ,而对t PA无显著影响 ;用卡维地洛干预后 ,PAI 1显著降低 ,t PA含量无明显改变。

Objective: To investigate the effects of tumor necrosis factor Alpha(TNF?α) and carvedilol on endothelial cell secreting tissue plasminogen activator(t?PA) and plasminogen activator inhibitor (PAI?1). Methods: Cultivated ECV304 cells were stimulated with TNF?α(5? 10? 25? 50? 100 ng/ml)or TNF?α plus carvedilol(20? 50? 100? 200 nmol/L)for 24 h. Tissue?type plasminogen activator (t?PA) and plasminogen activator inhibitor?1 (PAI?1) in the supernatant of the cell culture were measured by enzyme linked immunosorbent assay (ELISA). Results: The concentrations of t?PA had no statistically significant changes whether the ECV304 cells were stimulated by TNF?α or carvedilol.But PAI?1 was significantly increased by TNF?α and decreased by carvedilol. Conclusions: TNF?a and carvedilol have no effect on t?PA released from ECV304, but PAI?1 can be increased by TNF?α and decreased by carvedilol . TNF?α or carvedilol plays a role in the regulating of fibrinolysis.