糖尿病大鼠牙槽骨中肿瘤坏死因子-αmRNA表达与骨密度变化相关性研究

A Correlation Investigation Between Bone Density and mRNA Expression of TNF-α in Alveolars with Experimental Diabetic Rats

目的 研究糖尿病大鼠牙槽骨中肿瘤坏死因子 (TNF α)mRNA表达与骨密度变化之间的关系。方法 SD大鼠随机分成正常组与糖尿病组。实验第 8周 ,应用RT PCR技术检测大鼠牙槽骨中TNF αmRNA表达 ,并对牙槽骨进行X线片、骨密度、病理切片检查。结果 糖尿病组大鼠牙槽骨骨密度较正常组降低 ,TNF αmRNA表达高于正常组。结论 糖尿病组大鼠有骨质疏松倾向 ,而TNF

Objective To study the correlation between bone density and mRNA expression of TNF-α in the alveolars with experimental diabetic rats.Methods The SD rats were randomly divided into two groups:normal rats (Group N) and diabetic rats (Group D). After 8 weeks' study, TNF-α mRNA expression in the alveolr were measured by quantitative reverse transcription polymerase chain reaction(RT-PCR), respectively. In addition, radiograph, bone density and pathology were examined.Results In diabetic rats, the alveolar bone density decreased, and mRNA expression of TNF-α increased.Conclusion It was suggested that the alveolar bone in rats with diabetes mellitus had a tendency to develop osteoporosis. It might be related to increased TNF-α mRNA expression.