毫米波辐射兔肾区对庆大霉素所致急性兔肾损伤的影响

Effect of millimeter wave irradiation on gentamycin-induced nephrotoxicity in rabbits

目的 研究毫米波辐射对庆大霉素所致家兔急性肾损伤的影响 ,寻求防治药物诱发肾损伤的新方法。方法 将 16只家兔分为治疗组 (A组 ,共 7只 ) ,对照组 (B组 ,共 7只 )及正常组 (C组 ,共 2只 )。A、B两组每天给予庆大霉素 (GM)肌肉注射 ,连续注射 7d ;同时A组在实验第 2天即采用毫米波辐射右肾区 ,频率3 5 .75GHz ,波长 8.4mm ,功率密度 3 .5mW /cm2 。B组则不给予相应处理。分别于实验前、后测定各组家兔尿素氮 (BUN) ,血肌酐 (Scr) ,2 4h尿量及肌酐清除率 (Ccr)等指标 ,实验结束后观察各组家兔双侧肾脏组织病理学改变。结果 与实验前比较 :A组BUN无明显改变 ,B组在第 10天时上升明显 ;组间比较发现A ,B两组在第 10天时差异有显著性意义。开始实验后 ,A组Scr较实验前稍降低 ,在第 10天时差异有显著性意义 ,B组在第 10天时上升明显 ;组间比较发现A ,B两组在第 7天时差异有显著性意义 ;第 10天时差异有极显著性意义。A组 2 4h尿量增加明显 ,在第 5天、第 10天较治疗前差异均有显著性意义 ,而B组 2 4h尿量于第 7天时降至最低。A组Ccr较实验前无明显改变 ,B组在第 10天时降低明显 ;组间比较发现两组间在各时间点均无显著性差异。病理学检查发现A组损伤程度较B组轻。

Objective To observe the effect of millimeter wave irradiation on experimental renal impairment in rabbits. Methods Sixteen rabbits were recruited and randomly divided into three groups (A, B, C). Group C served as control in the experiment. The renal impairment was induced by intramuscular injection of gentamycin with rats in groups A and B once daily for seven days consecutively. Simultaneously, the rabbits in group A were treated with millimeter wave at the 1st day after injection. Group B were given no other special treatment. BUN, Scr, Urine Volume/24h and Ccr were measured before experiment and at the 3rd, 5th, 7th, 10th days after injection. At the end of experiment, all rabbits were sacrificed for histopathologic examination. Results In group A the content of BUN was not significantly different when compared with that of pre-experiment,. In group B, BUN was significantly higher at the 10th day than that of pre-experiment. There was significant difference between the groups A and B at the 10th day. At the 10th day Scr was lower than that of pre-experiment in group A, but significantly higher than that of pre-experiment in group B. There was significant difference between the groups A and B at the 7th and 10th days after experiment. Urine volume/24h was increased significantly at the 10th day in group A, but decreased significantly in group B at the 7th day. Ccr presented an obvious changes at the 10th day in group B. Histopathologic examination revealed a slighter structural changes in the group A than that of group B. Conclusion Millimeter wave could improve renal function and reduce gentamycin-induced nephrotoxicity in rabbits. It indicated that the millimeter wave irradiation had the therapeutic and preventive effect on the experimental acute renal impairment in rabbits.