辅助受体CCR5、CCR2及细胞趋化因子SDF1基因多态性与HIV-1异性传播的关系

Impact of the polymorphisms of CCR5,CCR2 and SDF1 on HIV-1 heterosexual transmission

目的 进一步阐述CCR5、CCR2和SDF1基因多态性与HIV 1异性传播的关系。方法 通过PCR RFLP技术分析CCR5、CCR2和SDF1基因的多态性 ,继而分析基因多态性与HIV 1异性传播的关系。结果 在收集到的 70对异性配对病例中 ,未能在汉族人群发现CCR5基因缺失突变 ,维吾尔族人CCR5Δ32基因频率为 6 .5 % (6 92 ) ,未发现纯合突变。有暴露史而未感染HIV 1者CCR2 6 4I基因频率明显高于受暴露后感染病毒者 ,说明CCR2 6 4I对异性传播可能具有一定保护作用。对SDF1基因的多态性研究发现 ,将病毒传播给配偶的指标病例 (先感染HIV 1一方 )SDF1 3′A的基因频率高于未发生传播者 (较接近于统计学检验水准 ) ,SDF1 3′A似乎是危险因素。结论 CCR5Δ32对汉族人群的HIV 1异性传播无明显意义 ,CCR2 6 4I对HIV 1异性传播可能具有一定的保护作用 ,而SDF1 3′A则有可能是危险因素 ,但有必要扩大样本量对后二者作进一步的深入研究。

Objective To investigate the impact of the polymorphisms of CCR5, CCR2 and SDF1 on HIV-1 heterosexual transmission. Methods The polymorphisms of CCR5, CCR2 and SDF1 were displayed by PCR/RFLP and carefully assessed as influential factors on HIV-1 heterosexual transmission. Results The deletion of CCR5 in the coding region (CCR5Δ32) wasn′t found in Han Chinese in this cohort, but the frequency of CCR5Δ32 was 6.5%(6/92) in Uygur people (no allele of CCR5Δ32/Δ32). The data didn′t sound the role of CCR5Δ32 in HIV-1 heterosexual transmission in China. On the other hand, a higher frequency of CCR2-64I allele was observed in HIV-1 uninfected partners who were heterosexually exposed to HIV-1 positive index partners than those infected with HIV-1 ( P =0.164). However, a higher frequency of SDF1-3′A allele was found in HIV-1 positive index individuals with HIV-1 positive partners than those with negative partners ( P =0.163). Conclusion Heterozygous allele of CCR5 (CCR5 +/Δ32) is not substantially supposed to influence HIV-1 heterosexual transmission in China. CCR2-64I may play a role in protecting from HIV-1 heterosexual transmission, but SDF1-3′A seems to be a risk factor.