膀胱移行细胞癌血小板衍化内皮细胞生长因子和血管内皮生长因子mRNA表达的研究

Study on mRNA Expressions of Platelet-derived Endothelial Cell Growth Factor and Vascular Endothelial Growth Factor in Bladder Transitional Cell Carc inoma

目的 研究血小板衍化内皮细胞生长因子 (PD ECGF)和血管内皮生长因子 (VEGF)在膀胱移行细胞癌生长与发展中的作用。方法 用逆转录聚合酶链反应 (RT PCR)测定了 3 5例膀胱移行细胞癌和 6例正常膀胱粘膜的PD ECGF和VEGFm RNA水平。结果 肌层浸润性 (T2 4)和粘膜下浸润性 (T1)膀胱移行细胞癌中 ,PD ECGF和VEGFmRNA水平均明显高于正常膀胱粘膜 ;粘膜下浸润性 (T1)与非浸润性乳头状 (Ta)膀胱移行细胞癌相比 ,PD ECGFmRNA明显增高 ,而VEGFmRNA无明显变化 ;非浸润性乳头状 (Ta)膀胱移行细胞癌中仅见VEGFmRNA水平明显高于正常膀胱粘膜 ,而PD ECGFmRNA无明显变化。PD ECGF和VEGFmRNA水平升高与膀胱移行细胞癌的高分级有关。结论 PD ECGF在膀胱移行细胞癌的浸润过程中可能起着重要作用 。

Objective To investigate the role of platelet-derived endoth elial cell growth factor(PD-ECGF) and vascular endothelial growth factor(VEGF)m RNA expressions during the growth and development of bladder transitional cell c arcinoma.Methods Reverse transcriptase polymerase chain reacti on was used to analyze the PD-ECGF and VEGF mRNA expressions in 35 cases of bla dder transitional cell carcinomas and 6 cases of normal bladder mucosa.R esults PD-ECGF and VEGF mRNA levels were higher in Muscle- and lamina propria-invasive bladder transitional cell carcinomas than in normal bladder m ucosa.Lamina propria-invasive bladder carcinoma expressed higher PD-ECGF mRNA level than non-invasive papillary bladder transitional cell carcinoma,while t hey expressed similar VEGF mRNA level.Non-invasive papillary bladder transitio nal cell carcinoma showed increased VEGF mRNA level than normal bladder mucosa, while they showed similar PD-ECGF mRNA level.Enhanced PD-ECGF and VEGF mRNA e xpressions were alsoassociated with the increased grade of bladder transitional cell carcinoma.Conclusion These data suggest that PD-ECGF mRN A expression may play an important role during the tumor invasion in bladder tran sitional cell carcinoma The VEGF mRNA expression correlates with the tumor grow th as well as invasion in bladder transitional cell carcinoma.