侧脑室注射6-OHDA对寒冷应激诱导的大鼠血浆ACTH含量的改变

Changes of Plasma ACTH Level in Rats Induced by Cold Stress after Intracerebroventricular(i.c.v)6-OHDA

目的 观察侧脑室注射 6 OHDA对 4℃寒冷应激诱导的血浆ACTH含量的改变。方法 成年Wistar雄性大鼠 3 0只 ,随机分成 5组 (n =6) ,分别为空白对照组、寒冷应激组、6 羟基多巴胺 (6 OHDA)非应激组、6 OHDA +寒冷应激组、配药液 +寒冷应激组。所有动物处理后立即取静脉血用放射免疫法测定其血浆ACTH含量。结果 4℃寒冷应激 4h后血浆ACTH含量明显升高 ,与正常对照组相比有明显差异 (P <0 .0 1) :6 OHDA侧脑室注射后再寒冷应激 ,血浆ACTH含量不再明显升高。

Objective To observe the changes of plasma ACTH level in rats induced by cold stress after i.c.v 6- OHDA Methods The animals we used in this study are adult, male Wistar rats.divided into 5 groups(n=6):control,cold stresa,i.c.v 6-OHDA,i.c.v 6-OHDA+cold stress,i .c.v saline+cold stress.Plasma ACTH was measured by radioimmunoassay.Re sults Plasma ACTH level was increased significantly by cold stress, and this changes could be blocked after 6-OHDA i.c.v.administration.Co nclusion Cold stress may activate HPA axis via catecholaminergic system .