VEGF与大肠癌浸润、转移关系研究

Study of Correlation between the Vascular Endothelial Growth Factor with the Invasiveness and Metastasis of Colorectal Carcinoma

目的 :探讨血管内皮生长因子 (VEGF)的表达与大肠癌浸润、转移的关系。方法 :采用免疫组化染色和RT PCR对 6 2例大肠癌进行了检测。结果 :6 2例大肠癌免疫组化 2 9例VEGF表达阳性 ,阳性率 4 6 8% ;RT PCR组 2 6例VEGF表达阳性 ,阳性率 4 2 0 %。肿瘤局限于黏膜层及黏膜下层和肌层、浆膜下层时 ,VEGF表达无明显差异 (P >0 0 5 ) ,但当肿瘤侵犯到浆膜层和浆膜外时 ,VEGF蛋白表达阳性率明显增高 (P <0 0 1 )。淋巴转移组的VEGF阳性表达显著高于未发生淋巴转移组(P <0 0 1 )。两种实验方法比较无统计学差异 (P >0 0 5 )。结论

Objective:To investigate the expression of vascular endothelial growth factor(VEGF) in colorectal carcinoma and their relationships with the invasiveness and metastasis of human colorectal carcinoma.Methods:The expression of VEGF was measured in 62 colorectal carcinoma samples with s p immunohistochemical technique and RT PCR ( reverse transcription polymerase chain reaction)Results:In immunohisto chemical technique the positive rate of VEGF is 46 8%.In RT PCR method the positive rate of VEGF is 42 0%.The positive rate of VEGF in colorectal cancer was correlated to the depth of invasiveness (P<0 01). The positive rate of VEGF was higher in the metastatic group than in the non metastatic group.There is a significant difference between two groups(P<0 01). However, there was no statistical difference between the two experimental methods.Conclusion:VEGF plays a key role in the depth of invasiveness and metastasis.