HSP70与PCNA在中耳胆脂瘤中的表达与意义

The Expression and Signiffcance of Heat Shock Protein 70 and Proliferating Cell Nuclear Antigen Protein in Human Middle Ear Cholesteatoma

目的通过分析热休克蛋白(heat shock protein,HSP)70和增殖细胞核抗原(proliferating cell nuclearantigen,PCNA)蛋白表达的相关性探讨HSP 70在中耳脂瘤病理过程中的可能作用。方法用HSP 70和PCNA单克隆抗体采用SP免疫组织化学方法对21例中耳胆脂瘤石蜡标本进行研究,11例正常外耳道皮肤做为对照。结果与正常外耳道皮肤相比,HSP70和PCNA相关蛋白在中耳胆脂瘤的表达均显著增强,均为P<0.01,二者的表达指数有一定相关性,r=0.568,P<0.01。结论 HSP 70在中耳胆脂瘤的异常表达说明其在胆脂瘤的发生、发展过程中可能有重要作用。HSP 70表达增多的同时胆脂瘤上皮细胞增生程度加重,提示HSP 70可能参与诱导上皮细胞增殖。

Purpose To evaluate the possible role of HSP 70 in cholesteatoma of the middle ear by inwestigating the correlationship between the expressions of HSP 70 and PCNA proteins. Methods All of the cholesteatoma tissue specimens ( n = 21) were examined with immunohistochemical SP method, using monoclonal antibodies of HSP 70 and PCNA,the normal external ear canal skin ( n = 11) used as the control. Results There was a significant sataistical difference of expressions of HSP 70-positive ( P < 0.01) and PCNA-positive cells (P < 0.01) between cholesteatoma epithelium and normal external ear canal skin. The expression index of HSP 70 protein in the epithelium of cholesteatoma was significantly correlated with that of PCNA (r= 0.568, P < 0.01) . Conclusion High density of HSP 70 in the epithelium of cholesteatoma suggested an important role of HSP 70 in the clinical development of cholesteatoma. Cell proliferation was accompanied by production of HSP 70. It seems that cell proliferation might be induced by increasing the synthesis of HSP 70.