促甲状腺激素受体在甲状腺相关眼病眼外肌原位表达的研究(英文)

In situ expression of thyrotropin receptor in the extraocular muscles of patients with thyroid-associated ophthalmopathy

目的研究甲状腺相关眼病(thyroid-associated ophthalmopathy, TAO)患者眼外肌组织中促甲状腺激素受体(Thy-rotropin receptor, TSHR)的表达,确定TSHR在TAO发病机制中的作用。方法收集手术治疗的12例严重的TAO患者的眼外肌组织标本及10例健康人意外死亡1 h内取出的眼外肌组织标本,4%中性甲醛固定24 h,石蜡包埋组织,作连续切片,采用免疫组织化学链霉卵白素·过氧化物酶连接法对12例严重TAO患者及10例健康人的眼外肌石蜡组织切片进行光学显微镜观察。结果TSHR在眼外肌肌束膜、细胞膜及少量胞浆中表达,12例TAO患者中有10例检测出TSHR蛋白,阳性检出率为83.3%,正常对照组未检测出TSHR蛋白的表达。结论TSHR蛋白TAO患者眼外肌组织中有表达,而在正常个体中没有表达,证实TAO患者球后眼外肌组织中存在有活性的TSHR蛋白,提示TSHR可能作为交叉抗原在TAO发病机制中起重要作用。

Objective To investigate the expression o f thyrotropin receptor (TSHR) in the extraocular muscles (EOM) of the eyes of p atients with thyroid-associated ophthalmopathy (TAO). Methods EOM specimens were obtained from 12 patients with severe TAO and 10 healthy subjects within 1 hou r after accidental death and the paraffin-embedded sections were ob-served by st reptavidin-peroxidase (SP) immunohistochemical methods. Result The expression of TSHR was observed in the perimysium, cytomembrane and cytoplasm in 10 of the 1 2 TAO specimens, accounting for a TSHR expression rate of 83.3% in the EOM of T AO patients. No TSHR protein was found in the healthy subjects. Conclusion High TSHR expression in the EOM of TAO patients indicates that as a common antigen b etween the orbit and thyroid, TSHR play a key role in the patho-genesis of TAO.