大肠侧向发育型肿瘤原代培养细胞的电镜观察

Electron microscopic observation of primary cultured laterally spreading tum or cell line

目的研究大肠侧向发育型肿瘤(LST)细胞经原代培养的细胞株的超微结构特点。方法将1例经肠镜切除的直肠LST细胞进行原代培养建株,然后进行扫描电镜及透射电镜观察,并与直肠腺癌及正常粘膜上皮细胞超微结构进行对照。结果LST原代培养的细胞表面被覆大量微绒毛,胞浆内有较多溶酶体、线粒体和吞噬颗粒,核异型性明显,核仁巨大,异型核沟。结论LST原代培养细胞具有高度恶性倾向。

Obj ec tive To observe the microscopic characteristics of laterally spreading tumor (LS T) cell line in primary cul-ture. Methods The cells isolated from a rectum LST s pecimen obtained by endoscopic mucosal resection was primary cul-tured, followed by observation with scanning and transmission electron microscope in comparison with the cells of adenocar-cinoma and normal mucosa of the rectum. Results Scan ning and transmission electron microscopes both revealed numerous microvilli co vering the surface of the LST cells, and the cytoplasm contained large quantity of lysosomes, mitochondria and phagosomes. Obviously heterogeneous cell nuclei were present with abnormal nuclear fossa and huge nucleoli. Conclusion The cultu red LST cells are highly malignant.