摘要
将柔红霉素C-14羟化酶基因与酪氨酸酶基因启动子和红霉素抗性基因启动子串联连接,克隆到变铅青链霉菌TK24中进行表达。经过SDS-PAGE蛋白电泳、CO结合差光谱分析结果证明,重组克隆表达的羟化酶分子量约45 kD、重组的蛋白量约占细胞总蛋白的12％,并具有细胞色素P-450的特征。
Daunorubicin C-14 hydroxylase gene (doxA), ligated with two tandem promoters for tyrosinase gene and erythromycin-resistance gene respectively, was cloned into Streptomyces lividans TK24 for expression of doxA gene. The results by SDS-PAGE and carbon monoxide-binding difference spectra analysis showed that the clone expressed C-14 hydroxylase whose molecular weight was about 45 kD, accounted for a 12 % of total proteins in the cells and possessed the cytochrome P-450 character.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2004年第1期13-15,共3页
Chinese Journal of Pharmaceuticals
基金
上海市自然科学基金(项目编号:01ZF14060)
