苯那普利对阿霉素肾病大鼠肾皮质Smad3、Smad7表达的影响

The effect of benazepril on the expression of renal cortical Smad3,Smad7 of the rats with adrimycin-induced nephropathy

目的 :评估苯那普利对阿霉素肾病大鼠肾皮质中Smad3、Smad7表达的影响。方法 :建立阿霉素肾病大鼠模型 ,药物组予苯那普利 10mg·kg-1·d-1灌胃。分别于第 4、7周取肾皮质 ,通过RT PCR半定量分析Smad7mRNA表达 ,通过光镜、电镜观察肾脏病理改变 ,用免疫组化法测定转化生长因子 β1(TGF β1)、Smad3、Smad7蛋白表达。结果 :Smad3、Smad7主要表达于肾小管上皮细胞 ,肾病组Smad7mRNA、蛋白的表达随病变加重而上调 ,Smad3蛋白表达下调 ,而苯那普利可抑制Smad7的上调和Smad3的下调。结论 :在阿霉素肾病大鼠肾皮质的小管病变进展中 ,Smads起着重要作用。

Objective To investigate the effect of benazepril on the expression of renal cortical Smad3,Smad7 of rats with Adriamycin nephropathy. Methods The rats were injected Adrimycin to create the model of Adriamycin nephropathy.The rats of benazepril treated group were delivered daily by gavage with 10 mg·kg -1 benazepril. The level of renal cortical Smad7 mRNA was examined by RT PCR at the forth week and the seventh week respectively. The localization and expression of Smad3, Smad7 protein were examined by immunohistochemistry staining. Results The staining for Smad3, Smad7 was presented in tubular epithelial cells. The expression of Smad7 mRNA,protein upregulated in the rats of nephrosis group, and the expression of Smad3 protein downregulated.Benazepril can repress the upregulation of Smad7 and the downregulation of Smad3. Conclusion The Smads play an important role during the development of tubular lesions of the renal cortex of the rats with Adriamycin nephropathy. Benazepril can regulate the expression of Smads, thereby protecting renal function.