VEGF、MMP-2与TIMP-2在大肠癌中的表达

Study on the expression of the inhibitor of VEGF,MMP-2 and TIMP-2 in colorectal carcinoma

目的 研究VEGF、MMP 2与TIMP 2在大肠癌组织中的表达、相互关系及与肿瘤生物学行为的相关性。方法 对 5 4例大肠癌患者的手术切除之癌组织和癌周正常组织 ,应用免疫组织化学方法检测其VEGF、基质金属蛋白酶MMP 2和组织金属蛋白酶抑制剂TIMP 2的表达情况 ,探讨两者之间的相互关系及其与肿瘤生物学行为的相关性。结果 VEGF、MMP 2与TIMP 2这三种蛋白在肿瘤组织中表达水平明显高于在正常组织中的表达。这三种蛋白的表达呈正相关 ,且表达水平与组织类型、组织分化程度、淋巴结转移、临床分期和五年生存率有统计学相关性 (P <0 .0 5 )。结论 大肠癌中VEGF、MMP 2与TIMP 2的表达 ,可能是癌肿发生、生长和浸润转移的重要因素 ,可作为判断肿瘤恶性程度的重要指标。

Objective To investigate the expression of VEGF, MMP-2 and TIMP-2 and the relationship between these three proteins, as well as the relationship between the expressions of these proteins and the biological behavior of carcinoma. Methods Immunohistochemical method was used to detect the expression of VEGF, MMP-2 and TIMP-2 in the carcinoma tissues and normal tissues around the foci. The relationship between these three proteins, together with the relationship between these proteins and the biological activity of carcinoma was further investigated. Results The expressions of VEGF and MMP-2 were high in carcinoma tissues while low in normal tissues. As for P33 ING1 , the case is to the contrary. There is correlation between the expressions of these proteins. And there is also statistical correlation between the expressions of these protein and the tissue type, tumor differentiation, metastasis of lymph node, Dukes stage, and five-year survival. Conclusion The high expressions of VEGF, MMP-2 and TIMP-2 in carcinoma increase may be important factors involved in carcinogenesis, development, invasion and metastasis, which can be used as predictors of malignant behavior of colorectal carcinoma.