大肠癌和大肠腺瘤COX-2和Bcl-2的基因表达及其意义

Expression of COX-2 and Bcl-2 genes in colorectal carcinoma and adenoma

目的 探讨COX 2及Bcl 2基因在大肠癌和大肠腺瘤组织中的表达及其意义。方法 应用免疫组化ABC法检测 2 8例大肠癌、2 8例大肠腺瘤和 10例正常黏膜中COX 2及Bcl 2表达 ,应用TUNEL法检测细胞凋亡。结果 在大肠癌、大肠腺瘤及正常黏膜中COX 2的阳性表达分别为 82 1%、85 7%和 0 0 %。Bcl 2的阳性表达分别为 75 0 %、78 6%和 2 0 0 %。大肠癌与腺瘤的COX 2及Bcl 2表达均无显著性差异 (P >0 0 5) ,但均显著高于正常黏膜 (P <0 0 1)。大肠癌中凋亡指数明显高于大肠腺瘤 (χ2 =8 80 ,P <0 0 5) ,COX 2和Bcl 2的表达均与大肠腺瘤及大肠癌的细胞凋亡指数呈显著负相关 (腺瘤P <0 0 1;癌P <0 0 5) ,两者均与临床病理特征无相关性 (P >0 0 5) ,大肠癌高、中、低分化各组间及不同Dukles分期 (A、B期和C、D期 )各组间凋亡指数无显著性差异 (P>0 0 5)。结论 COX 2和Bcl 2在大肠腺瘤及大肠癌中表达增强 ,从而抑制了细胞凋亡 。

ObjectiveTo investigate the mechanism of CO X-2 and Bcl-2 and genes in carcinogenesis of colorectal carcinoma and adenoma.Methods The expression of COX-2 and Bcl-2 were detected by i mm umohistochmically in colorectal cancinomas and 28 colorectal adenomas and 10 nor mal colorectal tissue.Apoptosis cells were detected by TUNEL. ResultsThe positive expression of COX-2 were 75 0%、78 6% a n d 0 0% respectively and the positive expression of Bcl-2 were 71 4%、39 3% a nd 20.0% respectively in colorectal caicinomas、adenomas and normal tissue.Expressi on of COX-2 and Bcl-2 was not significant difference in colorectal carcinomas an d adenomas (P>0 05).But expression of both were higher than normal tissue (P<0 01).Apoptosis index was much higher in colorectal caicinomas than in colorectal adenomas(χ 2=8.80, P<0 05) .The expressions of COX-2 an d Bcl-2 were reversely correlated to apoptosis index(ademomas,P<0 01;Carcinoma s,P<0 05).Their expressions were not associated with clinical pathological features of colorectal carcinoma.Apoptosis index was not associated with clinic al pathological features of colorectal carcinomas(P>0 05) ConclusionOverexpression of COX-2 and Bcl-2 in colorectal ad en oma and carcinoma may indirectly promote the carcinogenesis of colorectal cancer as an important gene inhibiting cell apoptosis,COX-2 and Bcl-2 play an import ant role in tumor development and progression.