槲皮素对胃癌细胞SGC-7901和BGC-823生长的影响

Effects of quercetin on proliferation of gastric cancer lines SGC-7901 and BGC-823

目的 观察槲皮素对胃癌细胞SGC 790 1和BGC 82 3生长和增殖的影响。方法 以台盼蓝拒染法计数胃癌细胞的生长抑制率 ,荧光显微镜了解凋亡的发生 ,流式细胞术检测细胞周期变化。结果 台盼蓝拒染法计数显示槲皮素抑制SGC 790 1和BGC 82 3细胞增殖的作用明显 ,呈浓度和时间依赖性 ,槲皮素处理 48h后的Ic50为 14 12 μm(SGC 790 1)和 2 8 13 μm(BGC 82 3 )。形态学检测显示出细胞凋亡的特征变化 ,流式细胞仪检测表明经 10～ 2 0 μm/L的槲皮素处理 ,SGC 790 1细胞周期阻滞于G0 /G1期 ,BGC 82 3细胞周期阻滞于S期。结论 槲皮素能抑制胃癌细胞的生长并诱导其发生凋亡 。

ObjectiveTo investigate the inhibition effects of quercetin on human gastric cancer lines SGC-7901 and BGC-823.MethodsCulturing gastric cancer cell line SGC-7901 and BGC- 82 3 cells in vitro,inhabiting gastric cancer cell with quercetin,the number of vi a ble cells was ascertained by trypan blue dye exclusion test.Morphological change s of apoptotic cells were observed by fluorescent microscopy,cell cycle were ana lysed by flow cytometry.ResultsQuercetin had a significant in h ibition on growth and proliferation of human gastric cancer line SGC-7901 and B G C-823 in vitro in a concentration-dependent manner.Fluorescent microscopy prov i ded evidence that apoptosis occurred in the gastric cancer cells of treatment gr oups.FCM analyses showed that quercetin caused an increase in SGC-7901 cells in the G 0/G 1 phase and decrease in the G 2/M phase of the cell cycle and cau se d an increase in BGC-823 cells in the S phase.ConclusionsQue rce tin markedly inhibited in a concertration-dependent manner the production of ga stric cancer cells and is an efficient anti-cancer drug.