摘要
目的观察单核细胞趋化蛋白-1(MCP-1)、Ⅳ型胶原(Ⅳ-C)在链尿佐菌素诱发的糖尿病肾病(DN)大鼠肾组织中的表达;探讨洛汀新、来氟米特、来适可对DN大鼠肾组织中MCP-1、Ⅳ-C表达的影响。方法将大鼠随机分成五组:正常组(C组)、糖尿病肾病组(D组)、糖尿病肾病洛汀新治疗组(DL组)、糖尿病肾病来氟米特治疗组(DF组)和糖尿病肾病来适可治疗组(DK组)。6周后,用免疫组化方法测定各组肾组织中MCP-1、Ⅳ-C的表达。结果MCP-1在DN大鼠肾小球、肾小管中的表达增高。Ⅳ-C在DL组肾小球中的表达与C组比较差异无显著性,在各治疗组与D组肾小管中的表达差异有显著性(P<0.01)。结论 MCP-1、Ⅳ-C在DN大鼠肾组织中表达增高;洛汀新、来氟米特、来适可能下调MCP-1在DN大鼠肾小球、肾小管中的表达;洛汀新可明显下调Ⅳ-C在DN大鼠肾小球中的表达。
Objective To observe the expression of monocyte chemoattractant protein-1 (MCP-1) and collagen Ⅳ (Ⅳ-C) in renal tissue of diabetic nephropathy rats that was induced by streptozotozin (STZ). To investigate the effects of Lotensin、LEF and Lescol for the expression of MCP-1 and Ⅳ-C in renal tissue of diabetic nephropathy rats. Methods The rats were randomly divided into five groups: normal control group(group C),diabetic nephropathy group (group D) .diabetic nephropathy rats treated with Lotensin (group DL),diabetic nephropathy rats treated with LEF (group DF),diabetic nephropathy rats treated with Lescol (group DK). after 6 weeks. The expression of MCP-1 and Ⅳ-C in renal tissue was detected by immunohisto-chemistry. Results There was high expression of MCP-1 and Ⅳ-C in renal tissue of diabetic nephropathy rats. MCP-1 was decreased by Lotensin, LEF and Lescol (P<0. 01) in glomerulus and tubule, Ⅳ-C was same between DL and C group in glomerulus, however there was different between treated groups and D group. Conclusion There was high expression of MCP-1 and Ⅳ-C in renal tissue of diabetic nephropathy rats. This expression could be inhibited by Lotensin、LEF and Lescol.
出处
《贵州医药》
CAS
2004年第1期10-12,共3页
Guizhou Medical Journal
