人胚胎干细胞源性表皮样干细胞分化潜能

Study on the Differentiation Potential of Epidermal-like Stem Cells Derived from Human Embryonic Stem Cells

[目的]探讨人胚胎干细胞源性表皮样干细胞的分化潜能,为研究其分化的调控机制及寻找新的人皮肤组织工程种子细胞奠定基础。[方法]将人胚胎干细胞与人羊膜共培养4 d,定向诱导其分化为表皮样干细胞克隆,用胰酶消化后移植裸鼠皮下20 d、30 d及50 d。对移植后细胞的分化情况进行了形态学和免疫组织化学观察分析。[结果]人胚胎干细胞源性表皮样干细胞在裸鼠皮下20-30 d后,细胞分化为由单层或复层上皮样细胞构成的管状或泡状结构,它们可分别呈CEA和CK18阳性。种植50 d后,除上述结构外,可见角化复层扁平上皮、毛囊样、汗腺样及皮脂腺样等结构。[结论]研究结果提示人胚胎干细胞源性表皮样干细胞具有分化为角化复层扁平上皮及毛囊样、汗腺样和皮脂腺样等结构的潜能。

[Objective] To explore the differentiation potential of epidermal-like stem cells derived from human embryonic stem cells to lay a base for the study of its differentiation mechanism so as to seek new source to provided seed cells for skin engineering. [Methods] Human embryonic stem cells were coculture with human amnion for 4 d. The epidermal like stem cells clones formed on the superficial of amnion were digested by trypsine and transplanted into subcutaneous of nude mouse for 20 d, 30 d and 50 d, then the differentiation pattern of the donor cells will observed and estimated with morphological and immunohistochemical method. [Result]The grafts may differentiation into tubular or follicular like structures lined with simple or stratified epithelial like cells which expressed CEA and CK18 respectively after 20-30 d transplantation. Keratinized stratified squamous epithelium, and hair follicle likes, sweat gland likes and sebaceous gland likes structure were observed after 50 d transplantation. [Conclusion] Our present preliminary study suggested that the epidermal-like stem cells derived from human embryonic stem cells might have the differentiation potential into keratinized stratified quamouse epithelial and hair follicle like, sweat gland like and sebaceous gland like structure.