大鼠睾丸间质tau蛋白和微管结合蛋白表达的变化

Expression Changes of tau Protein and Microtubule-associated Proteins in Testis Intersitium of Rats

目的 :检查老龄和青年大鼠睾丸间质组织中tau蛋白和微管结合蛋白的表达情况 ,籍此评价老龄大鼠睾丸衰退的发生机制。 方法 :应用二步法免疫组化技术检查 6月龄SD雄性大鼠 (10只 ,青年组 )和 2 8月龄同品系大鼠 (10只 ,老龄组 )睾丸组织抗Tau蛋白和抗微管结合蛋白的抗体免疫反应情况。 结果 :老龄大鼠睾丸间质组织中tau蛋白免疫反应阳性细胞数明显高于青年组 (P <0 .0 0 1) ,而青年组大鼠睾丸间质组织中微管结合蛋白免疫反应阳性细胞数明显高于老龄组 (P <0 .0 1)。 结论

Objective: To examine the expression of the tau protein and microtubule-associated proteins in the testis interstitium of aged and young rats. Methods: Sprague-Dawley male rats were divided into a young group(6 months, n=10) and an aged group(28 months, n=10). The two steps immunohistochemistry method with the antibody against tau protein and MAP α was performed on the testis tissues of the rats . Results: The results showed that the immunoreactive cells of tau protein of the testis interstilial of the aged rats obviously increased(P< 0.001)than those of the young, while the immunoreactive cells of the microtubule-associated proteins decreased(P< 0.01)in the aged. Conclusion: The changes in the expression of the tau protein and microtubule-associated proteins may be related to the aging process of the testis.