survivin蛋白在前列腺癌中的表达及其与肿瘤细胞凋亡的关系

Expression of survivin Protein in Prostatic Carcinoma Tissues and Its Correlation with Apoptosis of Cancer Cells

目的 :探讨新的凋亡基因survivin蛋白在前列腺癌 (PCa)的表达及其与肿瘤细胞凋亡的关系。 方法 :采用免疫组化SP法和DNA原位未端标记TUNEL法分别测定 4 2例PCa组织及 10正常前列腺 (NP)组织中survivin蛋白的表达和细胞凋亡。 结果 :survivin蛋白在PCa中的阳性表达为 80 .5 9% ,与病理分级、临床分期和淋巴结转移密切相关 (P <0 .0 5 ) ,而NP中无阳性表达 ;PCa组织及NP组织中细胞凋亡指数 (AI)分别为 3.0 3± 1.33、1.0 7± 0 .77,其差异有显著意义 (P <0 .0 5 ) ;survivin蛋白的表达与细胞AI呈负相关 (r=- 0 .6 79,P <0 .0 0 1)。 结论 :survivin蛋白的异常表达而引起的细胞凋亡抑制 ,在PCa的发生、发展中起一定的作用 ;联合检测survivin蛋白和AI,有助于对肿瘤细胞的分化程度作出正确评价 。

Objective: To investigate the expression of survivin protein in the tissues of prostatic carcinoma and its correlation with apoptosis of cancer cells. Methods: Expression of survivin protein and apoptosis index(AI) were detected by immunohistochemical and terminal deoxynucleotidyl transterase-mediated dUTP biotin nich end labeling(TUNEL) technique in the tissues of 42 cases of prostatic carcinima (PCa) and 10 cases of normal prostate (NP). Results: Survivin prosteins were expressed in 34 of the 42 ( 80.59%) cases of PCa. The positive rate of survivin was strongly associated with pathological grades, clinical stages and lymphmetastasis in PCa(P< 0.05). In contrast, NP did not express survivin. Survivin protein expressioin was negatively correlated with AI in PCa(r= -0.679, P< 0.001). Conclusions: Apoptosis inhibition by survivin may participate in the onset and progression of PCa, and the detection of survivin protein and AI in PCa may help to evaluate the degree of cell diferentiation, decide therapeutic strategies and estimate prognosis.