Survivin在急性白血病患者的表达及意义

Expression and Clinical Significance of Survivin in Acute Leukemia

目的 探讨急性白血病 (AL)患者Survivin基因表达及其临床意义。方法 应用RT -PCR方法检测 43例初治急性白血病患者Survivin基因mRNA表达。结果 AL患者SurvivinmRNA表达阳性率为 69 77%( 3 0 /4 3 )。 18例急性淋巴细胞白血病 (ALL)和 2 5例急性非淋巴细胞白血病 (ANLL)患者SurvivinmRNA表达阳性率 (分别为 72 2 2 %和 68 0 %)均高于对照组 ( 3 3 3 3 %,P <0 0 5 )。半定量后进行方差分析 ,ALL、ANLL及完全缓解 (CR)组患者的SurvivinmRNA表达水平 (分别为 0 63± 0 12 ,0 66± 0 19,0 73± 0 15 )也高于对照组 ( 0 43± 0 0 7,P <0 0 5 )。ANLL患者SurvivinmRNA表达水平受骨髓白血病细胞百分比影响 (b =1 0 98,P =0 0 0 5 )。AL患者SurvivinmRNA表达与骨髓缓解 (BMR)率无关。结论 Survivin基因在AL患者高表达 。

Objective To study the expression and clinical significance of survivin in acute leukemia. Methods By using semi-quantitative RT-PCR technique, survivin gene expression was detected in 43 acute leukemia(AL). Results Survivin gene expression rate in the cells of AL patients at diagnosis was 69.77%(30/43). Survivin mRNA expression rate in both 18 acute lymphocytic leukemia (ALL) and 25 acute nonlymphocytic leukemia(ANLL) (72.22% and 68.0% respectively) was signficantly higher than that in control group (33.33%,P<0.05). Detected by semi-quatitative RT-PCR, survivin mRNA expression level in ALL, ANLL and CR patients (0.63±0.12,0.66±0.19, and 0.73±0.15 respectively) was also higher than that in control group (0.43±0.07,P<0.05, by one-way ANOVA). Survivin mRNA expression level in ANLL correlated with the percentage of leukemic cells in the bone marrow (b=1.098,P=0.005). However, there was no significant difference in bone marrow remission (BMR) rate between survivin-positive and survivin-negative AL patients (P>0.05). Conclusions Survivin gene was highly expressed in AL patients and could be a potential target for treatment of AL.