ICAM-1在移植心脏缺血再灌注损伤中的表达及意义

The Expression and Significance of ICAM-1 on Myocardial Reperfusion Injury after Cardiac Transplantation in Rats

目的 探讨细胞间黏附分子 -1(ICAM -1)在移植心脏缺血再灌注损伤中的作用及意义。方法 2 4例Wistar大鼠心脏移植模型随机分为对照组 (n =6)和实验组 (n =18)。对照组移植心脏缺血 60min无再灌注。实验组移植心脏缺血 60min后分别再灌注 3h、6h、2 4h。采用免疫组织化学和生化法检测心肌ICAM -1的表达及髓过氧化物酶 (MPO)水平。结果 实验组移植心脏再灌注后心肌ICAM -1表达明显高于对照组 (P <0 0 5 )。再灌注 6h实验组心肌MPO水平明显高于对照组 (P <0 0 1)。结论 I CAM

Objective To investigate the expression and significance of intercellular adhesion molecule-1 (ICAM-1) in graft reperfusion injury after cardiac transplantation in rats. Methods Twenty-four Wistar rats heterotopic cardiac transplantation models were randomly divided into control group (n=6) and experimental group (n=18). In control group, the ischemic period of the grafts was 60min without reperfusion. In experimental group, the grafts were respectively reperfused for 3h, 6h and 24h after 60min ischemic period. Immunohistochemical and biochemical techniques were used to detect myocardial ICAM-1 and myeloperoxidase (MPO). Results The expression of allograft ICAM-1 after reperfusion in experimental group was significantly higher than that in control group (P<0.05). The level of graft MPO at 6h after reperfusion in experimental group was significantly higher than that in control group (P<0.01). Conclusions ICAM-1 may play important roles in the reperfusion injury in cardiac graft.