摘要
观察过氧化体增殖物激活型受体α在衰老过程中表达差异 ,在分子水平上探讨衰老过程中出现脂质代谢异常的可能机制。选用SD年轻大鼠 (6～ 8周龄 )和老年大鼠 (2 4月龄 ) ,测定血清甘油三酯和总胆固醇水平 ,采用逆转录—聚合酶链反应检测大鼠肝脏过氧化体增殖物激活型受体α及其目标基因mRNA水平 ,用Western印迹法检测过氧化体增殖物激活型受体蛋白的表达。结果发现 ,与年轻鼠组比较 ,老年鼠组血清甘油三酯和总胆固醇水平升高 ,过氧化体增殖物激活型受体αmRNA及蛋白表达随年龄增长而减少 ,目标基因的表达也受年龄的影响。结果提示 ,衰老过程中过氧化体增殖物激活型受体α表达减少可能与老年脂质代谢异常有关。
Aim To investigate difference expression of peroxisome proliferator-activated receptor α (PPAR α) during aging and explore the molecular mechanism of perturbation in lipid metabolism during aging. Methods We used two-group SD rats to estimate the lipid level of young (6～8 weeks) and aged (24 months) groups. The levels of triglyceride (TG) and total cholesterol (TC) were examined. The levels of liver PPAR α and target genes mRNA were measured by reverse transcription-polymerse chain reaction (RT-PCR) and PPAR α protein were determined by western blotting respectively. Results The TG and TC level of the aged group increased significantly. The PPAR α mRNA and protein level of the aged group significantly decreased compared with the young group. Target gene levels were changed during aging. Conclusion The mechanism of lipid dysfunction during aging is probably associated with the decreased expression of PPAR α.
出处
《中国动脉硬化杂志》
CAS
CSCD
2003年第6期498-500,共3页
Chinese Journal of Arteriosclerosis
基金
国家重点基础研究发展规划 (G2 0 0 0 0 5 70 0 7) ( 973课题 )
