血小板源生长因子BB对人血管平滑肌细胞钙调素的影响

Effects of Platelet-Derived Growth Factor BB on Content of Calmodulin in Cultured Human Vascular Smooth Muscle Cells

为研究血小板源生长因子BB对培养的人血管平滑肌细胞钙调素的影响 ,采用培养的人血管平滑肌细胞 ,应用磷酸二酯酶法观察不同浓度的血小板源生长因子BB对钙调素含量变化的影响及其时间效应。结果发现 ,平滑肌细胞在进入细胞增殖周期后 ,细胞内钙调素含量逐渐增加 ,在 9h时出现一个短暂的高峰 ,随后细胞内钙调素含量逐渐下降。血小板源生长因子BB可促进钙调素含量的增加 ,并呈现出明显的浓度依赖关系 ,30 μg L血小板源生长因子BB作用最为显著。结果提示 ,血小板源生长因子BB可明显促进培养的人血管平滑肌细胞钙调素含量的增加 ,并存在着一定的量效依赖关系及时间反应性。

Aim To investigate the effects of platelet-derived growth factor-BB (PDGF-BB)on content of calmodulin in human vascular smooth muscle cells. Methods The human vascular smooth muscle cells were cultured and effects of PDGF-BB on content of calmodulin in human vascular smooth muscle cells were assayed at different time and different concentration by its ability to stimulating calmodulin dependent cyclic nucleotide phsphodiesterase in vitro. Results Calmodulin level of quiescent human vascular smooth muscle cells was increased in its cell cycles with a maximal response 9 h, and PDGF-BB could significantly increase content of calmodulin of human vascular smooth muscle cells in a dose-dependent manner, with a maximal response at a concentration of 30 μg/L. Conclusion PDGF-BB could significantly increase calmodulin level of quiescent human vascular smooth muscle cells in a dose-dependent manner.