水飞蓟宾对实验兔球囊血管损伤后内膜增生的影响

Effects of Silybin on Neointimal Proliferation of Rabbit Iliac Artery after Balloon Injury

探讨水飞蓟宾对兔球囊血管损伤后内膜增生的影响。将新西兰大白兔随机分为对照组 (n =6 )、小剂量水飞蓟宾组 [n =8,2 0mg (kg·d) ]、大剂量水飞蓟宾组 [n =8,4 0mg (kg·d) ]。用球囊导管对实验兔行髂动脉损伤 ,用药组于术前 3d分别用小剂量及大剂量水飞蓟宾 ,术后 2 8d取病变血管染色并免疫组织化学检查 ,以计算机图像分析系统分析血管内膜、中膜厚度和腔面积的变化 ,计算内膜增生细胞核抗原增殖指数。结果发现 ,小剂量水飞蓟宾对血管内膜和中膜厚度、腔面积、内膜增生细胞核抗原阳性细胞百分比无明显影响 (P >0 .0 5 ) ;大剂量水飞蓟宾使腔面积扩大、内膜厚度减少、内膜增生细胞核抗原阳性细胞百分比减少 (P <0 .0 5 )。提示水飞蓟宾能抑制血管内膜增生 ,有可能用于防治再狭窄。

Aim To evaluate the effect of silybin on neointimal proliferation of rabbits iliac artery after balloon injury. Methods 22 rabbits were randomly separated into 3 groups including control group, low-dose treatment group and high-dose treatment group. Balloon catheter was used to induce injury to the iliac artery of rabbits in treatment group. Tranilast was given to the rabbits in low-dose treatment group [20 mg/(kg·d)] and high-dose treatment group [40 mg/(kg·d)] once a day from 3 days before operation to 28 days after operation, and then local vessel was harvested for pathological analysis and immunohistochemical staining. Automatic image analysis system was used to detect the changes of intimal thickness, medical thickness, luminal area, and the proliferation index of proliferating cell nuclear antigen (PCNA). Results No significant difference was found between low-dose treatment group and control group in intimal thickness, medial thickness, luminal area and proliferation index of PCNA (P> 0.05 ). The intimal thickness was decreased in high-dose treatment group compared with control group (P< 0.05 ). The luminal area was significantly larger in high-dose treatment group than that in control group (P<0.05). The proliferation index of PCNA was significantly reduced in high-dose treatment group compared with control group (P<0.05). Conclusions Silybin significantly inhibit neointimal hyperplasia and the drug may be used to prevent restenosis.