冠心病与白细胞介素6基因-572C/G多态性的相关性

The Relationship between Interleukin-6 Gene -572C/G Polymorphism and Coronary Heart Disease

为了解冠心病与白细胞介素 6基因 - 5 72C G多态的相关关系 ,应用聚合酶链反应—限制性片长多态性分析方法测定了 139例冠心病患者和 16 8例正常对照者的白细胞介素 6基因 - 5 72C G多态性 ,并结合疾病临床谱、冠状动脉造影结果探讨了其与冠心病的相关关系。结果发现 ,- 5 72C G基因型和等位基因频率在两组间存在明显差异 ,冠心病组GG基因型和G等位基因频率均显著高于对照组 (P <0 .0 1) ;相对于CC基因型 ,暴露于CG基因型、GG基因型的相对危险度分别为 1.37(95 %CI:0 .86～ 2 .2 0 ,P >0 .0 5 )和 2 .85 (95 %CI:1.32～ 6 .13,P <0 .0 1) ,校正了血压、血糖和血脂等传统危险因素的影响后 ,GG基因型的相对危险度 (95 %CI:1.2 2～ 6 .6 5 ,P <0 .0 5 )仍然具有统计学意义 ;但 - 5 72C G多态性在稳定型心绞痛、不稳定型心绞痛、心肌梗死患者间以及在单支病变组、双支病变组、三支病变组间的分布均无明显差异 (P >0 .0 5 )。结果提示 ,白细胞介素 6基因 - 5 72GG基因型可能是中国汉族人群冠心病发病的易感基因之一。

Aim To study the association of interleukin-6 (IL-6) gene -572C/G polymorphism with coronary heart disease (CHD). Methods IL-6/-572C/G polymorphism was examined in 139 CHD patients and 168 healthy adults by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results -572CC, CG and GG genotype frequencies were 51.80%, 40.29% and 7.91% in CHD group, 63.10%, 35.71% and 1.19% in control group respectively. -572 G and C allele frequencies were 28.06%, 71.94% and 19.05%, 81.95% in two groups respectively. GG genotype and G allele were more frequent in patients than in controls (P<0.01). Compared with CC genotype, relative risk (RR) for CHD were 1.37 (95%CI: 0.86～2.20, P>0.05) for CG genotype and 2.85 (95%CI: 1.32～6.13, P<0.01) for GG genotype. After adjusting for classical risk factors, the effect of GG genotype remained statistically significant (RR=2.85, 95%CI: 1.22～6.65,P<0.05). But the distribution of IL-6/-572C/G polymorphism had no significant difference among patients with stable angina, unstable angina and myocardial infarction, among patients with single-vessel,two-vessel and there-vessel lesions too(P>0.05). Conclusions IL-6/-572C/G polymorphism was associated with susceptibility to CHD and GG genotypes might be a risk factor for CHD in Chinese Hans population.