淋巴细胞表面HLA-I类抗原的阻断

Camouflage of HLA-I Antigen in Lymphocyte Surface

为了研究阻断淋巴细胞表面HLA I类抗原与其相应抗体特异性结合的方法及效果 ,在 2 2℃和 pH 7.4PBS介质中采用甲氧基聚乙二醇 苯并三唑碳酸盐 (mPEG BTC) ( 12mmol/L)处理淋巴细胞。结果表明 ,经mPEG BTC处理后的淋巴细胞与相应HLA I类抗体的微量淋巴细胞毒试验为阴性。结论 :在 2 2℃和pH 7.4条件下mPEG BTC可以阻断淋巴细胞表面HLA I抗原与其相应抗体的特异性结合。

The objective of this study was to investigate the method and effect of blocking the specific reaction between lymphocyte HLA I antigen and its antibody. The lymphocytes were disposed with 12 mmol/L methoxypolyethelene glycol benzotriazol carbonate (mPEG BTC) in concentration gradient in PBS (pH 7.4) at 22℃. The effect of the modified lymphocytes was detected by microlymphocytotoxicity assay. The results showed that lymphocytes modified by mPEG BTC did not react with related HLA I antibodies in microcytotoxicity test. It is suggested that the specific reaction between HLA I antigen of lymphocyte and HLA I antibodies can be completely camouflaged by mPEG BTC in PBS (pH 7.4) under 22℃ room temperature.