粒系集落刺激因子对外周血T淋巴细胞亚群的影响及与CD34^+细胞动员效果的相关性

The Effect of Granulocyte Colony Stimulating Factor on T-cell Subsets in Peripheral Blood and Its Correlation with the Mobilization Effect of CD34^+ Cells

本研究观察粒系集落刺激因子 (G CSF)作为造血干细胞动员剂对外周血T淋巴细胞亚群的影响及与CD34+细胞动员效果的关系。对 2 6例行自体造血干细胞移植患者在G CSF动员前后收集外周血标本 ,用流式细胞术检测动员前后CD3+、CD3+CD4 +、CD3+CD8+、CD3+CD4 +CD8+及CD3+CD4 -CD8-细胞绝对数量的变化并与外周血CD34+细胞的动员效果进行相关性分析。结果表明 :G CSF动员后外周血CD3+、CD3+CD4 +、CD3+CD4 +CD8+及CD3+CD4 -CD8-细胞的绝对数量分别增加 2 .2 3,2 .6 2 ,2 .99及 10 .96倍 ,而CD3+CD4 -CD8+细胞的变化无统计学意义 (P =0 .2 4 3)。各亚群细胞的变化与CD34+细胞动员效果比较 ,仅CD3+CD4 -CD8-细胞的变化与CD34+细胞动员效果间具有良好的相关性 ,r =0 .796 ,P =0 .0 0 0。结论 :G CSF将造血干细胞由骨髓动员到外周血的同时 ,使外周血中T细胞亚群的绝对数量发生不同程度的变化。在各T淋巴细胞亚群中CD3+CD4 -CD8-细胞的增加与CD34+细胞的动员效果间具有统计学意义的相关性。

The objective was to observe the effect of G CSF as a mobilizer of hematopoitic stem cells on the absolute counts of T cell subsets in peripheral blood and their relevance with the mobilized CD34 + cells. The examples of peripheral blood from 26 patients performed of autologous stem cell transplantation were taken before and after mobilization by G CSF . Flow cytometry was used for detecting CD3 +, CD3 +CD4 +, CD3 +CD8 +, CD3 +CD4 +CD8 + and CD3 +CD4 -CD8 - cells. Concurrently, their correlations with mobilized CD34 + cells in peripheral blood were compared. The results showed that after the mobilization by G CSF, the amounts of CD3 +, CD3 +CD4 +, CD3 +CD4 +CD8 + and CD3 +CD4 -CD8 - cells in peripheral blood increased by 2.23, 2.62, 2.99 and 10.96 fold respectively, but that of CD3 +CD4 -CD8 + cells was nearly no changed (P=0.243). The correlation coefficient of CD3 +CD4 -CD8 - cells and mobilized CD34 + cells was 0.796, (P=0.000) and no correlation with other T cell subsets. It was concluded that when CD34 + cells were mobilized by G CSF from bone marrow to peripheral blood, the absolute counts of the peripheral T cell subsets got changed. The increase of CD3 +CD4 -CD8 - cells had correlated with mobilization effect of CD34 + cells into peripheral blood.