兔唇创口愈合中肌成纤维细胞变化的实验研究

The Experimental Study of Myofibroblastic Pathologic Variation in Rabbits Wound Healing.

目的 :在动物模型中观察肌成纤维细胞的形态学变化 ,探讨肌成纤维细胞在创口愈合和瘢痕形成过程中的作用。方法 :选用同一子代纯种新西兰白兔 4 0只 ,采用Millard’s唇裂修复术 ,制造出创伤愈合瘢痕模型 ,分别在术后 7d、2 1d、30d、6 0d、90d进行肉眼观察和瘢痕体积测量 ,切取上唇肉芽和瘢痕组织在光镜和电镜下进行病理观察。结果 :肉眼观察兔唇创口愈合在不同时期有不同的变化。测量瘢痕体积的变化 ,在术后 30d ,瘢痕收缩较为明显 ,与 7d、90d时有显著意义 (P <0 .0 1) ,在不同时段MFB数量在 30d时与其他时段比较有显著意义 (P <0 .0 1) ,呈正态分布。光电镜下观察 ,术后 7d未出现肌成纤维细胞 ,2 1d可见少量 ,术后 30d表现得十分活跃 ,6 0d后又逐渐减少 ,90d时基本消失。结论 :瘢痕体积的缩小 ,与肌成纤维细胞的参与及胶原收缩、溶解有关。肌成纤维细胞是创口愈合和瘢痕形成过程中的一过性细胞 。

Objective:This purpose is observed myofibroblastic phohotoloyic variation in animal model,and to analyze functions of myofibroblasts between wound healing and formed of scar.Methods:fourty purel stors New Zealand rabbits were experimented that repaired uplips cleft by Millard's surgeny procedurce ,finally scar models were creacted. The volume of scar and granulation tissues were measured at 7, 21, 30, 60, 90 days postoperatively. The scaring tissue and granulation were harvested . The pathologic changes of scar were observed using the light microscopes and electronic microscopes on myofibroblasts.Results:The surface of scars was observed by naked eyes. The scars was swollen at 24 h postoperative. The wound healed on 7d postoperative. The scars was contracted and stiffen on 21d postoperative, soften and redden 30 days postoperative. Histopathological examination revealed the amount and vitalicatin of myofibroblasts were vival on 30 day postoperative. It was developmented on 21 day postoperative, and was vanished on 90day postoperative.Conclusions:To wrinke of scar volume corropinsh with taked part in myofibroblasts and collague contract and regeneral. Myofibroblasts were anatuactally cells in wound healing and formed of scar.thrie were play a impartment role forshapen of extracellar mitrax and scar contract.