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LRH-1/hB1F and HNF1 synergistically up-regulate hepatitis B virus gene transcription and DNA replication 被引量：6

LRH-1/hBlF and HNFl synergistically up-regulate hepatitis B virus gene transcription and DNA replication
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摘要 Enhancer Ⅱ(ENII)is one of the critical cis-elements in the Hepatitis B Virus(HBV)genome for the hepatic viral gene transcription and DNA replication.The liver-specific activity of ENII is regulated by multiple liver-enriched transcription factors,including LRH-1/hB 1 F,HNF 1,HNF3β,HNF4 and C/EBP.Knowledge on the interplay of these important factors is still limited.In this study,we demonstrate a functional synergism between the orphan nuclear receptor LRH-1/hB 1F and the homeoprotein HNF1 in up-regulating the liver-specific activity of ENII.This synergism is sufficient for initiating the viral gene transcription and DNA replication in non-hepatic cells.We have defined the activation domains in hB 1F and HNF 1 that contribute to the synergism.We further show that hB 1F and HNF1 can interact directly in vitro and have mapped the domains required for this interaction. Enhancer Ⅱ (ENⅡ) is one of the critical crs-elements in the Hepatitis B Virus (HBV) genome for the hepatic viral gene transcription and DNA replication. The liver-specific activity of ENII is regulated by multiple liver-enriched transcription factors, including LRH-1/hBlF, HNF1, HNF3β, HNF4 and C/EBP. Knowledge on the interplay of these important factors is still limited. In this study, we demonstrate a functional synergism between the orphan nuclear receptor LRH-1/hBlF and the homeoprotein HNF1 in up-regulating the liver-specific activity of ENII. This synergism is sufficient for initiating the viral gene transcription and DNA replication in non-hepatic cells. We have defined the activation domains in hB1F and HNF1 that contribute to the synergism. We further show that hB1F and HNF1 can interact directly in vitro and have mapped the domains required for this interaction.
作者 YANNINGCAI QINGZHOU YUYINGKONG MEILI BENOITVIOLLET YOUHUAXIE YUANWANG
机构地区 StateKeyLaboratoryofMolecularBiology OncogenicVirusesUnit.DepartmentofBiotechnology
出处 《Cell Research》 SCIE CAS CSCD 2003年第6期451-458,共8页 细胞研究（英文版）
基金 supported by the National Natural Science Foundation of China(30100088) High Technology Research and Development Project(2001-AA221261) Basic Research Program from Ministry of Science and Technology(G1999054105) supported by a Qi Ming Xing program(01QA14046)from Shanghai Science and Technology Committee
关键词 乙肝病毒 DNA复制 基因转录 增强子 LRH-1/hBlF, HNFl, HBV, ENII, synergism.
分类号 R373.21 [医药卫生—病原生物学]
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参考文献29

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同被引文献238

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  • 2Joachim Lupberger,Eberhard Hildt.Hepatitis B virus-induced oncogenesis[J].World Journal of Gastroenterology,2007,13(1):74-81. 被引量：57
  • 3Yan Cheng,Stephane Guindon,Allen Rodrigo,Lin Ying Wee,Masafumi Inoue,Alex J V Thompson,Stephen Locarnini,Seng Gee Lim.Cumulative viral evolutionary changes in chronic hepatitis B virus infection precedes hepatitis B e antigen seroconversion[J]. Gut . 2013 (9)
  • 4Clara Ramírez,Josep Gregori,Maria Buti,David Tabernero,Sílvia Camós,Rosario Casillas,Josep Quer,Rafael Esteban,Maria Homs,Francisco Rodriguez-Frías.A comparative study of ultra-deep pyrosequencing and cloning to quantitatively analyze the viral quasispecies using hepatitis B virus infection as a model[J]. Antiviral Research . 2013 (2)
  • 5Silvia Mirandola,Giada Sebastiani,Cristina Rossi,Emanuela Velo,Elke Maria Erne,Alessandro Vario,Diego Tempesta,Chiara Romualdi,Davide Campagnolo,Alfredo Alberti.Genotype-specific mutations in the polymerase gene of hepatitis B virus potentially associated with resistance to oral antiviral therapy[J]. Antiviral Research . 2012 (3)
  • 6Chun-Hui Yan,Cheng-Yu Zhao,Hai Ding,Ya-Qin Peng,Peng-Yuan Jin,Ling Yan,Hui Zhuang,Tong Li.Hepatitis B virus basal core promoter mutations A1762T/G1764A are associated with genotype C and a low serum HBsAg level in chronically-infected HBeAg-positive Chinese patients[J]. Antiviral Research . 2012 (2)
  • 7Sajad Shahmoradi,Yousef Yahyapour,Mahmood Mahmoodi,Seyed Moayed Alavian,Zeinab Fazeli,Seyed Mohammad Jazayeri.High prevalence of occult hepatitis B virus infection in children born to HBsAg-positive mothers despite prophylaxis with hepatitis B vaccination and HBIG[J]. Journal of Hepatology . 2012 (3)
  • 8European Association for the Study of the Liver.EASL Clinical Practice Guidelines: Management of chronic hepatitis B virus infection[J]. Journal of Hepatology . 2012 (1)
  • 9S.‐Y. Ko,H.‐B. Oh,C.‐W. Park,H. C. Lee,J.‐E. Lee.Analysis of hepatitis B virus drug‐resistant mutant haplotypes by ultra‐deep pyrosequencing[J]. Clinical Microbiology and Infection . 2012 (10)
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Cell Research
2003年 第6期

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