HPLC法测定复方降压平片中利血平的含量

Determination of reserpine in compound hypotensive tablet by HPLC

目的 :建立高效液相色谱法测定复方降压平片中利血平的含量。方法 :采用 C1 8(2 0 0 mm× 4 .6 mm,5 μm)色谱柱 ,乙腈 -水 (4 1:5 9)用高氯酸调节 p H值至 (2 .15± 0 .0 5 )为流动相 ,检测波长 2 6 8nm。结果 :利血平在 4 .0 0～ 16 .0 0μg/ml浓度范围内与峰面积呈良好线性关系 (r =0 .9996 ) ,平均回收率为 10 0 .5 % ,RSD =0 .34% (n =9)。结论 :该方法简便、准确。

Object: To establish an HPLC method for the determination of reserpine in compound hypotensive tablet (Beijing Hypotensive No.0). Methods: The chromatographic system consisted of C18 column and mobile phase of acetonitrilewater( adjusting the pH to 2.15±0.05 with perchloric acid ) (41∶59). The detection wavelength was set at 268 nm. Results: There was a good linear of reserpine over the range of 4.00～16.00μg·ml1(r=0.9996). The average recovery was 100.5% and RSD was 0.34% (n=9). Conclusion: This method was simple, accurate and reliable.