干化学法和湿化学法对尿淀粉酶检测的比较

The comparison of the dry and wet chemical methods for urine amylase

目的 分析干化学法和湿化学法测定患者样本尿淀粉酶结果的相关性及对两种方法进行偏倚评估。方法 依据美国国家临床实验室标准化委员会 (NCCLS)EP9 A文件 ,每天取临床样本 5份 ,分别用干化学和湿化学两种方法测定样本尿淀粉酶活力 ,共测定 9天 ,记录检验结果 ,进行离散点的检查 ,计算线性方程和相关系数并进行偏倚估计。结果 干化学法和湿化学法测定患者样本尿淀粉酶的结果具有很好的相关性 ,r =0 993。偏倚估计时样本尿淀粉酶活力为 180U/L、 4 90U/L和 980U/L ,干化学法和湿化学法测定结果的预期相对偏倚分别为 15 8%6 6 %和 3 7%。结论 干化学和湿化学法测定患者样本尿淀粉酶的结果具有很好的相关性 ,但也存在一定的差异 ,其比例误差是 0 0 12 ,恒定误差是 2 6 2 9U/L。随着样本尿淀粉酶活力的增加 。

Objective To analyze the correlativity of the dry and wet chemical methods for urine amylase (AMY) of patient samples and estimate the bias between two methods. Methods According to the procedure described by the NCCLS approved guideline,45 patient samples were analyzed in 9 operating days,each patient sample was analyzed in duplicate using both dry and wet chemical methods,the data was recorded and the separate spots were tested.The linear equation and the coefficient of correlation were calculated as well as the bias between two methods. Results In testing the patient's urine AMY,there was better correlativity between the dry and wet chemical methods,r=0.993.The expected relative bias between two methods was 15.8% at 180 U/L 6.6% at 490 U/L and 3.7% at 980 U/L for urine AMY. Conclusion Although the correlativity is good between the dry and wet chemical methods for urine AMY of patient samples,there are some differences:the proportional error is 0.012,the constant error is 26.29 U/L.In determing the patient's urine AMY,the relative bias between the dry and wet chemical methods goes down when the activity of urine AMY is increased.