摘要
目的 探讨中国鼠疫菌株生化表征的遗传变异与分型地位。方法 采用平皿 -单菌落法 ,实验观察我国17个生态型共 3 3 6株鼠疫菌酵解麦芽糖、鼠李糖、阿胶糖、甘油及脱氮反应的变化。结果 发现松辽平原、滇闽居民区和滇西纵谷区 3个生态型部分菌株对甘油、阿胶糖、麦芽糖酵解有变异 ,可同时出现酵解和不酵解的单菌落 ,而且特性较稳定 ;其余 14个生态型鼠疫菌单菌落酵解能力与过去资料报道一致无明显变化 ;根据实验结果可将中国鼠疫菌分为 12个生化型。结论 我国鼠疫菌对糖酵解特性稳定 ,具有一定的分型意义 ,滇闽居民区与滇西纵谷区生态型鼠疫菌株可能源于一个祖先。
Objective To explore the genetic variation and typing significance of biochemical characters of Yersinia pestis isolated from P. R. China. Methods To observe the change of 336 strains of. 17 ecotypes with the single colony of every strain for ferments L-maltose, L-Rhamnose, L-Arabinose, and Glycerol and reduces KNO 3 by plate method. Results There were 3 ecotypes, the Songliao Plain, the Dianmian Residential Area and the Dianxi Vertical Vale with different colonies in fermenting Glycerol, Arabinose and maltose, respectively. The remainder was steady and consistent with the past experiments. 17 ecotypes of Yersinia pestis could be divided into 12 biochemical types according to the study results. Conclusion Yersinia pestis strains are steady to ferment the above carbohydrates, which have a certain sense in Yersinia pestis typing. The Dianmian Residential Area and the Dianxi Vertical Vale are likely to derive from the same ancestor.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2003年第6期460-462,共3页
Chinese Journal of Vector Biology and Control
基金
科技部基础性工作专项资金
关键词
鼠疫菌
生化特征
流行病学
遗传变异
分型地位
Yersinia pestis
Biochemical characters
Epidemiological significance