摘要
目的 :探讨不同DNA提取方法对石斛属DNA质量及分子标记结果的影响。方法 :对提取的DNA进行紫外光谱分析、总DNA电泳及RAPD分析。结果 :不同方法提取的DNA质量及产率存在显著差异 ,对RAPD结果影响很大。结论 :石斛属植物在DNA提取前加入无CTAB缓冲液抽提多糖等杂质 ,可获得较高质量的DNA。
Objective: To explore the quality of DNA with three methods of DNA extraction and the influence on RAPD-PCR. Method: the electrophoresis of total DNA ,UV spectrophotometry and RAPD analysis were carried out on DNA extracted from three different methods. Result: The DNA concentration and yields were different,which greatly influenced the result of RAPD-PCR. Conclusion: The higher quality DNA from Dendrobium can be obtained with the method of CTAB-free extraction medium before total DNA was isolated.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2003年第12期1129-1131,共3页
China Journal of Chinese Materia Medica
基金
重庆市科委应用基础课题 (2 0 0 0 672 12 )
关键词
总DNA
提取
鉴定
石斛属
紫外光谱
Dendrobium total
DNA
RAPD
electrophoresis
UV spectrophotometry
