金黄色葡萄球菌对小鼠产生一氧化氮及一氧化氮合酶的影响

EFFECTS OF STAPHYLOCOCCUS AUREUS ON THE PRODUCTION OF NITRIC OX IDE AND NITRIC OXIDE SYNTHASE IN MICE

目的 :探讨金黄色葡萄球菌对小鼠产生一氧化氮 (NO)及一氧化氮合酶 (NOS)的影响 ,以进一步研究 NO及 NOS在抗感染免疫中的作用。方法 :将不同剂量的金黄色葡萄球菌注入小鼠腹腔 ,10 d后取小鼠血清和腹腔巨噬细胞培养上清 ,用硝酸还原酶法检测其 NO的含量 ,同时测定血清中 NOS的水平及抗金黄色葡萄球菌抗体的效价。结果 :金黄色葡萄球菌注射小鼠后 ,血清中 NO及 NOS的水平明显高于对照组 (P<0 .0 1) ,各组间两两比较亦差异有显著性 (P<0 .0 1)。腹腔巨噬细胞培养上清 NO的水平明显高于对照组 (P<0 .0 1) ,但不同剂量实验组之间差异无显著性 (P>0 .0 5)。结论 :金黄色葡萄球菌可引起小鼠血清中 NO、NOS升高 ,NO及

Objective: To investigate the effects of Staphy lo coccus aureus (S.aureus) on the production of Nitric Oxide (NO) and Nitric Ox ide Synthase(NOS) in mice. Methods:Different does of S.aureus w ere injected int o mice's intraperitoneal. Serum and the supernatants of cell cultures peritoneal macrophage were collected, then concentration of NO was detected. The level of NOS and antibody of S.aureus in the mice's serum were assayed. Results : The level of NO、NOS and antibody of S.aureus in the serum of tested were signif icantly higher (P<0.01). There appeared an obvious increasing on the concen tration of NO in the supernatants of cell cultures mouse peritioneal macrophage (P<0.01),but the between level of NO in two tested groups was no significa n tly difference (P>0.05). Conclusion: S. aureas could c ause increasing t he production of NO and NOS of serum in mice. It was possible that increase of N O and NOS played an important role in the immunity of resistance to microbes.