细菌自动培养仪厌氧培养的临床应用

CLINICAL APPICATION OF ANAEROBIC CULTURE WITH AUTOMATED MICROBIAL CUL TURE SYSTEM

目的 :探讨用细菌自动培养仪作厌氧培养的临床应用价值。方法 :选用 mini VITAL 全自动荧光血培养仪检测常见临床标本 5472份 (其中血液 4869瓶、胸腹水 2 40瓶、脓腔及组织穿刺液 175瓶、脑脊液12 2瓶、骨髓液 2 3瓶、胆汁及其引流液 2 1瓶、其他 2 2瓶 )厌氧培养结果 ,对其阳性检出率、检出细菌种类和时间、假阳性以及药敏结果进行综合分析和评估。结果 :设定收瓶时间为 5d,厌氧培养的阳性检出率为2 5.51% (其中胆汁 76.19% ,脓汁 57.14 % ,血液 2 5.2 8% ,胸腹水 14 .17% ,骨髓 13 .0 4% ,脑脊液 5.74% ) ;共检出各种感染菌 3 3属 77种 ,专性厌氧菌、微需氧菌和兼性厌氧菌的比例为 4.8∶ 1.0∶ 94.2 ;专性厌氧菌的平均检出时间为 (2 6.13± 2 0 .59) h;兼性厌氧菌的检出时间厌氧株比需氧株平均延长 -0 .54～ 4.7h;假阳性率 0 .16% ;药敏结果厌氧株的耐药率普遍较需氧株低。结论 :仪器厌氧培养简便、快速、安全、有效 ,是临床常见标本检测厌氧菌值得推荐使用的方法 ;

Objective:To study the evaluation of c li nical application of anaerobic culture with automated microbial culture system.Methods:A total of 5 472 samples were detected by anaerobic cul ture with automated microbial cluture system,its included 4 869 samples of blood and 240 samples of pleural and abdominal fuluid,175 samples of pus and 122 sam ples of cerebrospinal fluid and 23 samples of bone marrow,21 samples of bile and 22 samples of the others.To assay and evaluate the positive detection rate and the types of bacteria recovered,and the time of positive detection and the false positive rate,and the result of bacterial drug resistance.Results:1 396 stains(25 51%) were isolated by anaerobic cultur e.Thereinto,the positive rate of bile was 76 19%,blood was 25 28%,pleural and abdominal fluid was 14 17%,bone marrow was 13 04%,cerebrospinal fluid was 5 74% These isolates included 33 species and 77 types of bacteria.These ratio of obligate anaerobes and microaerobes and facultative anaerobes were 4 8∶1 0∶9 4 2 The average detection time of obligate anaerobes was (26 13±20 59)h,the average detection time of anaesobic culture of facultative anaerobes more than aerobic culture was -0 54～4 7 h The false positive rate was 0 16% Anaero bic strains of drug resistance lower than aerobic strains.Conclusions:The method of anaerobic culture by automated microb i al culture system was a better measure of bacterial anaerobic culture for clinic al specimens.Applied both of anaerobic culture and aerobic culture of anaerobes, the positive detection rate has been raised.