聚乙二醇修饰重组人干扰素α-2b修饰产物的初步分析研究

Primary analytical study on the PEG-modified rhIFNα-2b

目的 对聚乙二醇(PEG)修饰重组人干扰素α-2b(rhIFNa-2b)的修饰产物进行了初步分析研究。方法以单甲氧基聚乙二醇甲酸琥珀酰亚胺酯对rhIFNα-2b进行修饰,修饰产物以Superdex 75 Highload制备型凝胶色谱柱进行分离、纯化,SDS-PAGE鉴定各组分,采用Mariner电喷雾飞行时间质谱对单修饰IFNα-2b(Inono-PEG-IFNα-2b)进行分析。结果 分别获得了纯化的mono-PEG-IFNα-2b和多修饰IFNα-2b(poly-PEG-IFNα-2b)。ESI-TOF质谱也证明mono-PEG-IFNα-2b分子量比rhIFNα-2b多5 000左右;紫外光谱分析表明,PEG修饰产物的结构没有发生改变。结论获得了纯化的PEG修饰产物。

Purpose To study PEG-modified rhIFNα-2b. Methods rhIFNα-2b was modified with SCmPEG, the products were isolated and purified with Superdex 75 Highload column and identified with SDSPAGE. ESI-TOF-MS was applied to analyze mono-PEG-IFNα-2b. Results Purified mono-PEG-IFNα-2b and poly-PEG-IFNα-2b were obtained, and molecular weight of mono-PEG-IFNα-2b was approved to be about 5000 than IFNα-2b by ESI-TOF-MS. UV spectrum demonstrated that The structure of the modified products did not change. Conclusion Purified mono-PEG-IFNα-2b and poly-PEG-IFNα-2b were obtained.