Svate-Ⅲ对原代培养的大鼠皮层神经元钙激活钾通道作用

Effect of Svate-Ⅲ on K^+ channel activated by Ca^(2+) in primary culture rat cortex neuron

目的 观察Svate-Ⅲ对原代培养新生SD大鼠皮层神经元上钙激活钾通道作用。方法 采用膜片钳单通道记录技术。结果 Svate-Ⅲ在胞外可使钙激活钾通道开放概率增加,开放时程延长,在一定范围内表现出浓度依赖性。结论 Svate-Ⅲ可激活钙激活钾通道,可能对神经元有直接保护作用。

Purpose To observe the effect of Svate-Ⅲ on K^+ channel activated by Ca^(2+) in primary culture fetal SD rat cortex neuron. Methods The patch clamp technique of single channel recordings including cell-attach and inside-out mode was used. Results Svate-Ⅲ can activate the Ca^(2+)-activated K^+ channel, and can increase its probability of channel opening and lengthen the duration of channel opening. It demonstrates the dependence on concentration in some range. Conclusion Svate-Ⅲ can activate the Ca^(2+)-activated K^+ channel, and maybe has a protective effect on neurons.