摘要
目的 :研究急性脊髓损伤后神经细胞凋亡的分布特别及其意义。方法 :Wistar雌性大白鼠 5 4只 ,使用改良Allen法制作急性脊髓损伤模型 ,分别于术后 1、 4、 8、 2 4、 72h、 7、 14及 2 1d处死取材 (每时间点n =6)。应用HE染色及凋亡细胞原位末端标记法 (TUNEL)对脊髓组织进行标记。结果 :损伤后 4h ,在损伤段及邻近段可见末端标记的阳性神经细胞 ,损伤段灰质中阳性细胞数 2 4h达高峰 ,72h白质中阳性胶质细胞数量达高峰。相邻节段阳性细胞数量在 72h达高峰。灰质中神经元及胶质细胞均有阳性表达 ,但以胶质细胞为主。结论 :脊髓损伤后神经细胞凋亡是继发损伤期的重要病理变化 ,并有其时相和空间分布特点。
Objective: To study the characteristics of neural cells with apoptosis after acute spinal cord injury.Methods:The spinal cord injury of the Wistar rats was induced with Allen's way by a 10g×2.5cm impact on the posterior T_ 8,9 spinal cord.Rats were sacrificed at 1,4,8,24,72 hours and 7,14,21 days after injury(n=6 for each time point).Three segments of every spinal cord were cut for morphological studies,including hematoxylin and eosin staining and the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) methods.Results:TUNEL-positive cells were found in the compression region and neighbor regions after 4h,with a maximum presence at 24h in the compressed segment,and labeled glial cells in white matter reached a peak at 72h.But the labeled cells in the neighboring segments reached a peak at 72h after injury.Both the neural and glial cells in gray matter were expressed TUNEL positive,especially the glial cell.Conclusions:Apoptosis of neural cells is an important morphological change in the secondary lesion period after spinal cord injury.
出处
《中国矫形外科杂志》
CAS
CSCD
2003年第24期1699-1700,共2页
Orthopedic Journal of China
