慢性硝酸镧染毒大鼠肝脏中镧沉积的研究

Study on lanthanum deposit in liver of rats chronically exposed to lanthanum nitrate at low dose

目的 探讨低剂量硝酸镧 [La(NO3) 3]的慢性毒性及镧在大鼠肝脏中的沉积情况。方法 连续用 0 1、0 2、2 0、10 0和 2 0 0mg kg的La(NO3) 3给大鼠灌胃 6个月后 ,应用透射电镜技术 (TEM)、X射线微区分析 (XMA)和电感耦合等离子体质谱法 (ICP MS)研究镧在大鼠肝脏中的沉积。结果 2 0 0mg kgLa(NO3) 3组肝细胞内电子密度高的致密体较多 ,大鼠肝脏中镧的含量 ( 3 890ng g蛋白 )与对照组 ( 3 0ng g蛋白 )相比 ,差异有非常显著意义 (P <0 0 1)。结论 镧可进入到肝细胞内 。

Objective To study deposit of lanthanum in rat liver.Methods Young Wistar rats were divided into six groups,which were fed at dose of 0^1,0^2,2^0,10^0,20^0 mg/kg lanthanum nitrate (La(NO-3)-3) respectively for six months.The metabolic accumulation of lanthanum(La)in rat liver were investigated by transmission electron microscope(TEM),X-ray microanalysis (XMA)and inductively coupled plasma-mass spectrometry(ICP-MS).Results The number of bodies containning high electronic dense gravel-like granules and lysosomes with dense bodies in hepatocytes increased gradually.The contents of La at dose of 20^0 mg/kg La(NO-3)-3( 3 890 ng/g protein)were higher than those of controls(30 ng/g protein,P<0.01).Conclusion La(NO-3)-3 can enter cells.The content of La in the liver increases regularly with increase of dose and time of drug delivery.