雄性棉铃虫和烟青虫对雌性信息素的触角电生理反应

EAG responses of the male cotton bollworm (Helicoverpa armigera) and the male oriental tobacco budworm (H.assulta) to female sex pheromones

利用触角电位图 (Electroantennogram ,EAG)技术 ,比较研究了二近缘种棉铃虫、烟青虫对其性信息素主要成分Z 11 16 :Ald、Z 9 16 :Ald的触角电生理反应。剂量反应曲线表明 ,对Z 11 16 :Ald ,棉铃虫和烟青虫均有明显的EAG反应 ,且随浓度的增加而增强 ,但棉铃虫比烟青虫的反应较强 ;对Z 9 16 :Ald ,烟青虫有很强的EAG反应 ,棉铃虫的反应则很弱 ;对Z 11 16 :Ald和Z 9 16 :Ald以 97∶3和 7∶93比例形成的混合物 ,棉铃虫、烟青虫均有EAG反应 。

Comparison of electroantennogram (EAG) responses between Helicoverpa armigera and H.assulta to female sex pheromone major components, (Z) 11 hexadecenal(Z 11 16:Ald) and (Z) 9 hexadecenal(Z 9 16:Ald) was investigated. The basal end of the isolated antennae was connected to the indifferent electrode, and the tip after the terminal segment removed was linked with the recording electrode. The antennae were stimulated by six dosages of sex pheromone components from 1 ng/μl to 10 5 ng/μl through a Pasteur pipette. The EAG signal was amplified by DC/AC amplifier and recorded by Spike software on a PC computer. The absolute EAG data were used for statistical analysis. The dose response curve showed that both H. armigera and H.assulta had high response amplitudes to Z 11 16: Ald, but that of H. armigera was higher than that of H. assulta from 10 2 ng/μl to 10 5 ng/μl. High spike amplitude was elicited in H. assulta by Z 9 16: Ald, but relative low response to Z 9 16: Ald in H. armigera was detected, this indicates that there is no Z 9 16:Ald sensillum on the H.armigera antennae. Both H. armigera and H. assulta had strong responses to mixtures of Z 11 16:Ald and Z 9 16:Ald with ratios 97∶3 and 7∶93, but with no significant difference between the two species. Results suggested that the EAG response of H. armigera and H. assulta could detect the single specific component of sex pheromones, but could not tell the difference of species specific pheromone blends.