虎杖的组织培养与快速繁殖

Tissue culture and rapid propagation of Reynoutria japonica

以虎杖茎段、叶柄、叶片为外植体探讨了愈伤组织诱导、分化和植株再生的条件,筛选出茎段生长培养基为1/2MS+BA1.0mg·L-1+KT0.5mg·L-1+NAA0.2mg·L-1,茎段、叶柄和叶片外植体愈伤组织诱导培养基为MS+BA1.0～2.0mg·L-1+KT0.2～0.5mg·L-1+NAA0.2～0.5mg·L-1或MS+BA2.0～3.0mg·L-1+KT0.2～0.5mg·L-1+2,4-D0.5mg·L-1;丛生芽诱导培养基为MS+BA2.0mg·L-1+KT0.5mg·L-1+IBA0.2mg·L-1+LH1000;不定根及根状茎诱导培养基为1/2MS+IBA0.2mg·L-1.

The stem, petiole and blade of Reynoutria japonica were used as explants and cultured on MS media containing different phytohormons. The optimum media of callus formation and shoot differentiation in vitro were determined. The medium of primary culture of stem segments was 1/2 MS + BA 1.0 mg·L^(-1)+ KT 0.5 mg·L^(-1)+ NAA 0.2mg·L^(-1). Calluses were induced on MS + BA 1.0～2.0 mg·L^(-1)+ KT 0.2～0.5 mg·L^(-1)+ NAA 0.2～0.5 mg·L^(-1)and MS + BA 2.0～3.0 mg·L^(-1)+ KT 0.2～0.5 mg·L^(-1)+ 2,4-D 0.5 mg·L^(-1). Adventitious buds were induced on MS + BA 2.0 mg·L^(-1)+ KT 0.5 mg·L^(-1)+ IBA 0.2 mg·L^(-1)+ LH 1000. Roots were induced on 1/2 MS + IBA 0.2 mg·L^(-1).