摘要
Blood samples were collected from chicken embryos at stage 11～15, and labeled with fluorescent dye PKH26. Primordial germ cells (PGCs) were then isolated from blood samples by nycodenz density gradient centrifugation. After PGCs were labeled and isolated, about 200 PGCs in one microliter were injected into the subgerminal cavity of quail blastoderm at stageⅩ. After 48 hours incubation, chicken PGCs were identified by fluorescent microscopy. Red fluorescence emitted from PKH26 labeled chicken PGCs was observed in the head, the heart and the developing gonadal anlage of quail embryos. The result suggests that chicken PGCs still keep migration ability after 56 hours.
Blood samples were collected from chicken embryos at stage 11～15, and labeled with fluorescent dye PKH26. Primordial germ cells (PGCs) were then isolated from blood samples by nycodenz density gradient centrifugation. After PGCs were labeled and isolated, about 200 PGCs in one microliter were injected into the subgerminal cavity of quail blastoderm at stageⅩ. After 48 hours incubation, chicken PGCs were identified by fluorescent microscopy. Red fluorescence emitted from PKH26 labeled chicken PGCs was observed in the head, the heart and the developing gonadal anlage of quail embryos. The result suggests that chicken PGCs still keep migration ability after 56 hours.
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2003年第6期868-872,共5页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金 (No .3 9980 0 3 0 )资助项目
国家"863"计划(No .2 0 0 1AA2 13 13 1)资助项目~~
关键词
鸡
鹌鹑
原始生殖细胞
生殖原基
荧光色素
禽类
Chicken, Quail, Primordial germ cells(PGCs), Gonadal anlage, Fluorescent dye
