摘要
Monoclonal antibodies raised against human sperm were used as a probe to look for specific human sperm antigen. By “ Western blotting” ,an 80 K dalton molecular weight protein was recognized by the monoclonal antibody, 21-2-4. By the SDS polyacrylamide gel electrophoresis, the 80 Kd was the major band of deoxycholate extract in which this protein could be isolated from other components of sperm by eleetro-elution from the gel slab. For the purpose of evaluating the function of 80 Kd in fertilization, the rabbit anti-80 Kd polyclonal antibodies were produced and immunoassays were performed. The TAT result showed that the agglutination in patterns of tail to tail and head to head were induced by incubating human sperm with rabbit anti-8OKd antiserum. The SIT result showed that the human sperm motility was strongly inhibited by rabbit anti-80 Kd antiserum. The immanofluorescence staining of human sperm incubated with rabbit anti-80 Kd antiserum showed very strong fluorescence on postaerosome and tail. These results suggest that 80 Kd is a specific sperm antigen which locates on the surface of human sperm and possibly plays an important role in fertilization.
