游离胆甾醇酶传感器的研制

Development of a Free Cholesterol Biosensor

本文初步研制了一种测定游离胆固醇的酶传感器。该传感器用于流动注射法的最佳工作条件:磷酸盐缓冲液作载液(pH=6.3,0.1mol/L),流动速度为0.5mL/min,温度为27℃,检测限为0.15mg/mL～1.14mg/mL,线性响应范围0.25～1.00mg/mL,斜率0.08μA·mL/mg,响应时间为0.5～2.0min,工作寿命15d。已将该传感器用于样品分析,取得初步满意的结果。

The determination of cholesterol is very important in clinical analysis. In this thesis, the development of a cholesterol oxidase biosensor is reported.Nylon mesh activated with dimethyl sulphate and reacted with lysine and glutaraldehyde, then cholesterol oxidase was reacted with GA and was immonilized on the nylon mesh. The nylon mesh with immobilized enzyme was fixed on an oxygen electrode. The optimized working conditions of the biosensor is : O.lmol/L phosphate buffer (pH6.3) as carrier solution, with a rate of 0.15-1.14mg/mL and the linear range is 0.25-1,00mg/mL, the slope is about 0.08μA.mL/mg. The response time of the sensor in linear range ≤2.5min and the life time is 15-day. This sensor has some advantages over those reported previously in working ranges and linear ranges.